PMID- 27270042
OWN - NLM
STAT- MEDLINE
DCOM- 20170612
LR  - 20220129
IS  - 1878-1551 (Electronic)
IS  - 1534-5807 (Print)
IS  - 1534-5807 (Linking)
VI  - 37
IP  - 5
DP  - 2016 Jun 6
TI  - Annexin A1 Tethers Membrane Contact Sites that Mediate ER to Endosome Cholesterol 
      Transport.
PG  - 473-83
LID - S1534-5807(16)30282-9 [pii]
LID - 10.1016/j.devcel.2016.05.005 [doi]
AB  - Membrane contact sites between the ER and multivesicular endosomes/bodies (MVBs) 
      play important roles in endosome positioning and fission and in neurite 
      outgrowth. ER-MVB contacts additionally function in epidermal growth factor 
      receptor (EGFR) tyrosine kinase downregulation by providing sites where the 
      ER-localized phosphatase, PTP1B, interacts with endocytosed EGFR before the 
      receptor is sorted onto intraluminal vesicles (ILVs). Here we show that these 
      contacts are tethered by annexin A1 and its Ca(2+)-dependent ligand, S100A11, and 
      form a subpopulation of differentially regulated contact sites between the ER and 
      endocytic organelles. Annexin A1-regulated contacts function in the transfer of 
      ER-derived cholesterol to the MVB when low-density lipoprotein-cholesterol in 
      endosomes is low. This sterol traffic depends on interaction between ER-localized 
      VAP and endosomal oxysterol-binding protein ORP1L, and is required for the 
      formation of ILVs within the MVB and thus for the spatial regulation of EGFR 
      signaling.
CI  - Copyright (c) 2016 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Eden, Emily R
AU  - Eden ER
AD  - UCL Institute of Ophthalmology, London EC1V 9EL, UK.
FAU - Sanchez-Heras, Elena
AU  - Sanchez-Heras E
AD  - UCL Institute of Ophthalmology, London EC1V 9EL, UK.
FAU - Tsapara, Anna
AU  - Tsapara A
AD  - UCL Institute of Ophthalmology, London EC1V 9EL, UK; Technical University of 
      Crete, 73100 Chania, Greece.
FAU - Sobota, Andrzej
AU  - Sobota A
AD  - Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland.
FAU - Levine, Tim P
AU  - Levine TP
AD  - UCL Institute of Ophthalmology, London EC1V 9EL, UK.
FAU - Futter, Clare E
AU  - Futter CE
AD  - UCL Institute of Ophthalmology, London EC1V 9EL, UK. Electronic address: 
      c.futter@ucl.ac.uk.
LA  - eng
GR  - 093445/Wellcome Trust/United Kingdom
GR  - G0801878/MRC_/Medical Research Council/United Kingdom
GR  - MR/M010767/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PL  - United States
TA  - Dev Cell
JT  - Developmental cell
JID - 101120028
RN  - 0 (Annexin A1)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Receptors, Steroid)
RN  - 0 (VAPA protein, human)
RN  - 0 (VAPB protein, human)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (oxysterol binding protein)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.1.3.48 (PTPN1 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
SB  - IM
MH  - Annexin A1/*metabolism
MH  - Biological Transport/drug effects
MH  - Cholesterol/*metabolism
MH  - Endocytosis/drug effects
MH  - Endoplasmic Reticulum/drug effects/*metabolism/ultrastructure
MH  - Endosomes/drug effects/*metabolism/ultrastructure
MH  - Epidermal Growth Factor/pharmacology
MH  - ErbB Receptors/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Lipoproteins, LDL/pharmacology
MH  - Multivesicular Bodies/drug effects/metabolism/ultrastructure
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 1/metabolism
MH  - Receptors, Steroid/metabolism
MH  - Transport Vesicles/drug effects/metabolism
MH  - Vesicular Transport Proteins/metabolism
PMC - PMC4906250
EDAT- 2016/06/09 06:00
MHDA- 2016/06/09 06:01
PMCR- 2016/06/06
CRDT- 2016/06/09 06:00
PHST- 2015/07/01 00:00 [received]
PHST- 2016/04/11 00:00 [revised]
PHST- 2016/05/09 00:00 [accepted]
PHST- 2016/06/09 06:00 [entrez]
PHST- 2016/06/09 06:00 [pubmed]
PHST- 2016/06/09 06:01 [medline]
PHST- 2016/06/06 00:00 [pmc-release]
AID - S1534-5807(16)30282-9 [pii]
AID - 10.1016/j.devcel.2016.05.005 [doi]
PST - ppublish
SO  - Dev Cell. 2016 Jun 6;37(5):473-83. doi: 10.1016/j.devcel.2016.05.005.