PMID- 27270865 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20180119 LR - 20180119 IS - 1096-9888 (Electronic) IS - 1076-5174 (Linking) VI - 51 IP - 6 DP - 2016 Jun TI - Surface acoustic wave nebulization device with dual interdigitated transducers improves SAWN-MS performance. PG - 424-9 LID - 10.1002/jms.3766 [doi] AB - We compared mass spectrometric (MS) performance of surface acoustic wave nebulization (SAWN) generated by a single interdigitated transducer (IDT) designed to produce a progressive wave (PW) to one with a dual IDT that can in theory generate standing waves (SW). Given that devices using dual IDTs had been shown to produce fewer large size droplets on average, we hypothesized they would improve MS performance by improving the efficiency of desolvation. Indeed, the SW-SAWN chip provided an improved limit of detection of 1 femtomole of peptide placed on chip making it 100x more sensitive than the PW design. However, as measured by high-speed image recording and phase Doppler particle analyzer measurements, there was only a 26% increase in the small diameter (1-10 microm) droplets produced from the new device, precluding a conclusion that the decrease in droplet size was solely responsible for the improvement in MS signal/noise. Given that the dual IDT design produced a more instantaneous plume than the PW design, the more likely contributor to improved MS signal/noise was concluded to be a higher ion flux entering the mass spectrometer for the dual IDT designs. Notably, the dual IDT device allowed production of much higher quality protein mass spectra up to about 20 kDa, compared with the single IDT device. Copyright (c) 2016 John Wiley & Sons, Ltd. CI - Copyright (c) 2016 John Wiley & Sons, Ltd. FAU - Huang, Yue AU - Huang Y AD - Deurion, LLC, Seattle, WA, USA. FAU - Heron, Scott R AU - Heron SR AD - Deurion, LLC, Seattle, WA, USA. FAU - Clark, Alicia M AU - Clark AM AD - Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. FAU - Edgar, J Scott AU - Edgar JS AD - Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA. FAU - Yoon, Sung Hwan AU - Yoon SH AD - Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD, USA. FAU - Kilgour, David P A AU - Kilgour DP AD - School of Science & Technology, Nottingham Trent University, Nottingham, UK. FAU - Turecek, Frantisek AU - Turecek F AD - Department of Chemistry, University of Washington, Seattle, WA, USA. FAU - Aliseda, Alberto AU - Aliseda A AD - Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. FAU - Goodlett, David R AU - Goodlett DR AD - Deurion, LLC, Seattle, WA, USA. AD - Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD, USA. LA - eng PT - Journal Article PL - England TA - J Mass Spectrom JT - Journal of mass spectrometry : JMS JID - 9504818 OTO - NOTNLM OT - SAWN OT - SAWN-MS OT - mass spectrometry OT - standing wave OT - surface acoustic wave nebulization EDAT- 2016/06/09 06:00 MHDA- 2016/06/09 06:01 CRDT- 2016/06/09 06:00 PHST- 2015/12/25 00:00 [received] PHST- 2016/03/13 00:00 [revised] PHST- 2016/03/21 00:00 [accepted] PHST- 2016/06/09 06:00 [entrez] PHST- 2016/06/09 06:00 [pubmed] PHST- 2016/06/09 06:01 [medline] AID - 10.1002/jms.3766 [doi] PST - ppublish SO - J Mass Spectrom. 2016 Jun;51(6):424-9. doi: 10.1002/jms.3766.