PMID- 27271316
OWN - NLM
STAT- MEDLINE
DCOM- 20171107
LR  - 20230203
IS  - 1935-3456 (Electronic)
IS  - 1933-0219 (Linking)
VI  - 10
IP  - 2
DP  - 2017 Mar
TI  - Composition and dynamics of the uterine NK cell KIR repertoire in menstrual 
      blood.
PG  - 322-331
LID - 10.1038/mi.2016.50 [doi]
AB  - Uterine natural killer (NK) cells are abundantly present in endometrium and 
      decidua. Their function is governed by interactions between killer cell 
      immunoglobulin-like receptors (KIRs) and cognate human leukocyte antigen (HLA) 
      class I ligands. These interactions have implications for reproductive success. 
      Whereas most uterine NK cells are known to express KIRs, little information is 
      available about KIR repertoire formation and stability over time. This is 
      primarily due to inherent difficulties in gaining access to human uterine tissue. 
      As endometrial immune cells are shed during menstruation, menstrual blood may 
      serve as a source for studies of KIRs on uterine NK cells. Here, we performed a 
      combined assessment of six inhibitory and activating KIRs on uterine NK cells 
      from paired menstrual and peripheral blood. Menstrual blood contained a high 
      frequency of uterine NK cells expressing KIRs. The uterine NK cell KIR 
      repertoires were markedly different from those in peripheral blood NK cells, 
      biased toward KIR2D-receptor expression, and formed independently of selection 
      conferred by cognate HLA class I molecules. Moreover, uterine NKG2C(+)self-KIR(+) 
      NK cell expansions were detected. Finally, the distinct KIR repertoires of 
      uterine NK cells were stable over multiple menstrual cycles. Our results provide 
      novel insight into KIR repertoire formation on human uterine NK cells.
FAU - Ivarsson, M A
AU  - Ivarsson MA
AD  - Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska 
      Institutet and Karolinska University Hospital, Stockholm, Sweden.
AD  - Department of Pathology, University of Cambridge, UK.
AD  - Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.
FAU - Stiglund, N
AU  - Stiglund N
AD  - Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska 
      Institutet and Karolinska University Hospital, Stockholm, Sweden.
FAU - Marquardt, N
AU  - Marquardt N
AD  - Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska 
      Institutet and Karolinska University Hospital, Stockholm, Sweden.
FAU - Westgren, M
AU  - Westgren M
AD  - Department of Clinical Science, Intervention and Technology, Karolinska 
      Institutet, Stockholm, Sweden.
AD  - Department of Obstetrics and Gynecology, Karolinska University Hospital, 
      Stockholm, Sweden.
FAU - Gidlof, S
AU  - Gidlof S
AD  - Department of Clinical Science, Intervention and Technology, Karolinska 
      Institutet, Stockholm, Sweden.
AD  - Department of Obstetrics and Gynecology, Karolinska University Hospital, 
      Stockholm, Sweden.
AD  - Department of Women's and Children's Health, Karolinska Institutet, Stockholm, 
      Sweden.
FAU - Bjorkstrom, N K
AU  - Bjorkstrom NK
AD  - Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska 
      Institutet and Karolinska University Hospital, Stockholm, Sweden.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160608
PL  - United States
TA  - Mucosal Immunol
JT  - Mucosal immunology
JID - 101299742
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KLRC2 protein, human)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (Receptors, KIR)
SB  - IM
MH  - Adult
MH  - Blood Cells/*immunology
MH  - Cell Proliferation
MH  - Female
MH  - Fertility
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Killer Cells, Natural/*immunology
MH  - Menstruation/*blood
MH  - Middle Aged
MH  - NK Cell Lectin-Like Receptor Subfamily C/metabolism
MH  - Receptors, KIR/*metabolism
MH  - Uterus/*immunology
MH  - Young Adult
EDAT- 2016/06/09 06:00
MHDA- 2017/11/08 06:00
CRDT- 2016/06/09 06:00
PHST- 2015/07/29 00:00 [received]
PHST- 2016/05/04 00:00 [accepted]
PHST- 2016/06/09 06:00 [pubmed]
PHST- 2017/11/08 06:00 [medline]
PHST- 2016/06/09 06:00 [entrez]
AID - S1933-0219(22)00646-8 [pii]
AID - 10.1038/mi.2016.50 [doi]
PST - ppublish
SO  - Mucosal Immunol. 2017 Mar;10(2):322-331. doi: 10.1038/mi.2016.50. Epub 2016 Jun 
      8.