PMID- 27273604
OWN - NLM
STAT- MEDLINE
DCOM- 20180411
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jun 8
TI  - Macrophage Migration Inhibitor Factor Upregulates MCP-1 Expression in an 
      Autocrine Manner in Hepatocytes during Acute Mouse Liver Injury.
PG  - 27665
LID - 10.1038/srep27665 [doi]
LID - 27665
AB  - Macrophage migration inhibitor factor (MIF), a multipotent innate immune 
      mediator, is an upstream component of the inflammatory cascade in diseases such 
      as liver disease. Monocyte chemoattractant protein-1 (MCP-1), a highly 
      representative chemokine, is critical in liver disease pathogenesis. We 
      investigated the role of MIF in regulating hepatocytic MCP-1 expression. MIF and 
      MCP-1 expression were characterized by immunochemistry, RT-PCR, ELISA, and 
      immunoblotting in CCl4-treated mouse liver and isolated hepatocytes. MIF was 
      primarily distributed in hepatocytes, and its expression increased upon acute 
      liver injury. Its expression was also increased in injured hepatocytes, induced 
      by LPS or CCl4, which mimic liver injury in vitro. MIF was expressed earlier than 
      MCP-1, strongly inducing hepatocytic MCP-1 expression. Moreover, the increase in 
      MCP-1 expression induced by MIF was inhibited by CD74- or CD44-specific siRNAs 
      and SB203580, a p38 MAPK inhibitor. Further, CD74 or CD44 deficiency effectively 
      inhibited MIF-induced p38 activation. MIF inhibitor ISO-1 reduced MCP-1 
      expression and p38 phosphorylation in CCl4-treated mouse liver. Our results 
      showed that MIF regulates MCP-1 expression in hepatocytes of injured liver via 
      CD74, CD44, and p38 MAPK in an autocrine manner, providing compelling information 
      on the role of MIF in liver injury, and implying a new regulatory mechanism for 
      liver inflammation.
FAU - Xie, Jieshi
AU  - Xie J
AD  - Department of Cell Biology, Municipal Laboratory for Liver Protection and 
      Regulation of Regeneration, Capital Medical University, Beijing 100069, China.
FAU - Yang, Le
AU  - Yang L
AD  - Department of Cell Biology, Municipal Laboratory for Liver Protection and 
      Regulation of Regeneration, Capital Medical University, Beijing 100069, China.
FAU - Tian, Lei
AU  - Tian L
AD  - Department of Cell Biology, Municipal Laboratory for Liver Protection and 
      Regulation of Regeneration, Capital Medical University, Beijing 100069, China.
FAU - Li, Weiyang
AU  - Li W
AD  - Department of Cell Biology, Municipal Laboratory for Liver Protection and 
      Regulation of Regeneration, Capital Medical University, Beijing 100069, China.
FAU - Yang, Lin
AU  - Yang L
AD  - Department of Cell Biology, Municipal Laboratory for Liver Protection and 
      Regulation of Regeneration, Capital Medical University, Beijing 100069, China.
FAU - Li, Liying
AU  - Li L
AD  - Department of Cell Biology, Municipal Laboratory for Liver Protection and 
      Regulation of Regeneration, Capital Medical University, Beijing 100069, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160608
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - *Autocrine Communication
MH  - Biomarkers
MH  - Carbon Tetrachloride/adverse effects
MH  - Chemical and Drug Induced Liver Injury/genetics/metabolism
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Disease Models, Animal
MH  - *Gene Expression Regulation
MH  - Hepatocytes/*metabolism
MH  - Liver Diseases/*genetics/*metabolism/pathology
MH  - Macrophage Migration-Inhibitory Factors/*metabolism
MH  - Mice
MH  - Phosphorylation
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
PMC - PMC4897699
EDAT- 2016/06/09 06:00
MHDA- 2018/04/12 06:00
PMCR- 2016/06/08
CRDT- 2016/06/09 06:00
PHST- 2016/02/15 00:00 [received]
PHST- 2016/05/24 00:00 [accepted]
PHST- 2016/06/09 06:00 [entrez]
PHST- 2016/06/09 06:00 [pubmed]
PHST- 2018/04/12 06:00 [medline]
PHST- 2016/06/08 00:00 [pmc-release]
AID - srep27665 [pii]
AID - 10.1038/srep27665 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jun 8;6:27665. doi: 10.1038/srep27665.