PMID- 272742
OWN - NLM
STAT- MEDLINE
DCOM- 19780426
LR  - 20031114
IS  - 0250-7870 (Print)
IS  - 0250-7870 (Linking)
VI  - 20
IP  - 6
DP  - 1977 Nov-Dec
TI  - Genetic manipulation in higher organisms. I. Goldfish ova as materials of 
      operation, mRNA mediated alteration of the liver specific isozymes.
PG  - 803-6
AB  - Both crucian and carp egg-mRNAs were found to mediate the transformation of 
      goldfish tail from the double (veil-shaped) to the single (fork-shaped). Livers 
      of these single tailed goldfish were tested for genetic change using lactate 
      dehydrogenase isozymes (LDH) as marker. Electrophoresis of liver extracts on 
      starch gel showed that the liver specific LDH-C4 isozymes of both the egg-mRNA 
      and liver-mRNA injected goldfish resembled the control goldfish except an 
      additional intermediate band. The migration distance of this new band matched the 
      third band of the hybrids (carp X goldfish). These findings lead to the 
      conclusion that carp egg-mRNA contains a fraction partly responsible for liver 
      development. Rat liver was found possessing the cathodally migrating C4 isozymes. 
      Injection of rat liver-mRNA into goldfish eggs resulted in the intensification of 
      the liver specific C4 isozymes. It appears therefore that the input informational 
      molecules (liver-mRNA) and the output molecular products (liver C4 isozymes) are 
      causally related. This causal relationship would pave the way for genetic 
      manipulation in higher organisms.
FAU - Niu, M C
AU  - Niu MC
FAU - Tung, T C
AU  - Tung TC
LA  - eng
PT  - Journal Article
PL  - China
TA  - Sci Sin
JT  - Scientia Sinica
JID - 8209876
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Carps/*genetics
MH  - Cyprinidae/*genetics
MH  - Female
MH  - Genetic Engineering
MH  - Goldfish/*genetics
MH  - Isoenzymes/*analysis
MH  - Kidney/enzymology
MH  - L-Lactate Dehydrogenase/*analysis
MH  - Liver/*enzymology
MH  - Ovum/*enzymology
MH  - RNA, Messenger/*analysis
MH  - Rats
EDAT- 1977/11/01 00:00
MHDA- 1977/11/01 00:01
CRDT- 1977/11/01 00:00
PHST- 1977/11/01 00:00 [pubmed]
PHST- 1977/11/01 00:01 [medline]
PHST- 1977/11/01 00:00 [entrez]
PST - ppublish
SO  - Sci Sin. 1977 Nov-Dec;20(6):803-6.