PMID- 27277337
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 2
DP  - 2016 Aug
TI  - Autophagic death induced by thermo‑chemotherapy in gastric cancer cells results 
      from the reactive oxygen species pathway.
PG  - 1210-8
LID - 10.3892/mmr.2016.5353 [doi]
AB  - Gastric cancer is the third leading type of cancer and has the third leading 
      cancer‑associated mortality in China. The mechanism of thermo‑chemotherapy in 
      gastric cancer cells remains to be elucidated. The present study aimed to 
      investigate the role of autophagic cell death in the thermo‑chemotherapy of 
      gastric cancer. The current study included four groups: An empty control group, a 
      hyperthermia group, a chemotherapy (oxaliplatin) group, and a thermo‑chemotherapy 
      group. Cell viability was analyzed by the MTS assay. Production of intracellular 
      reactive oxygen species (ROS) was quantified by flow cytometry. 
      Autophagy‑associated proteins, Beclin 1, microtubule‑associated protein 
      1A/1B‑light chain (LC3B) and mammalian target of rapamycin (mTOR), were 
      determined by western blot analysis. The results indicated that 
      thermo‑chemotherapy markedly increased intracellular ROS production, and 
      decreased mitochondrial membrane potential. The transmission electron microscopy 
      results indicated that thermo‑chemotherapy induced production of autophagic 
      bodies. In addition, thermo‑chemotherapy‑induced cell damage at the cellular and 
      animal levels indicated a notable increase in the expression of the 
      autophagy‑associated genes, LC3B and Beclin 1. A negative correlation between 
      mTOR expression and autophagy was also identified, which demonstrates that 
      thermo‑chemotherapy induces autophagic cell death by activating the 
      autophagy‑associated signaling pathways. The results of the present study 
      demonstrated that the ROS level is important in autophagic death of the gastric 
      carcinoma cells, and the increased ROS level, induced by thermo‑chemotherapy 
      treatment, induced autophagy in gastric carcinoma cells.
FAU - Wu, Yinbing
AU  - Wu Y
AD  - Treatment Center of Body Cavitary Thermo‑perfusion, Cancer Hospital of Guangzhou 
      Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Pan, Mingxin
AU  - Pan M
AD  - Second Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, Guangdong 510282, P.R. China.
FAU - Cui, Shuzhong
AU  - Cui S
AD  - Treatment Center of Body Cavitary Thermo‑perfusion, Cancer Hospital of Guangzhou 
      Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Ba, Mingchen
AU  - Ba M
AD  - Treatment Center of Body Cavitary Thermo‑perfusion, Cancer Hospital of Guangzhou 
      Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Chen, Zulong
AU  - Chen Z
AD  - Treatment Center of Body Cavitary Thermo‑perfusion, Cancer Hospital of Guangzhou 
      Medical University, Guangzhou, Guangdong 510095, P.R. China.
FAU - Ruan, Qiang
AU  - Ruan Q
AD  - Treatment Center of Body Cavitary Thermo‑perfusion, Cancer Hospital of Guangzhou 
      Medical University, Guangzhou, Guangdong 510095, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160527
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Organoplatinum Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 04ZR38536J (Oxaliplatin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - Autophagy/*drug effects/*radiation effects
MH  - Biomarkers
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects/radiation effects
MH  - Disease Models, Animal
MH  - Drug Resistance, Neoplasm
MH  - *Hot Temperature
MH  - Humans
MH  - *Hyperthermia, Induced
MH  - Inhibitory Concentration 50
MH  - Membrane Potential, Mitochondrial/drug effects/radiation effects
MH  - Mice
MH  - Organoplatinum Compounds/pharmacology
MH  - Oxaliplatin
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/*drug effects/*radiation effects
MH  - Stomach Neoplasms/drug therapy/genetics/*metabolism/pathology
MH  - Xenograft Model Antitumor Assays
EDAT- 2016/06/10 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/06/10 06:00
PHST- 2015/05/13 00:00 [received]
PHST- 2016/02/25 00:00 [accepted]
PHST- 2016/06/10 06:00 [entrez]
PHST- 2016/06/10 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - 10.3892/mmr.2016.5353 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Aug;14(2):1210-8. doi: 10.3892/mmr.2016.5353. Epub 2016 May 27.