PMID- 27280804
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20220330
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 25
DP  - 2016 Jun 29
TI  - Surface Chemistry of Nanoscale Mineralized Collagen Regulates Periodontal 
      Ligament Stem Cell Fate.
PG  - 15958-66
LID - 10.1021/acsami.6b04951 [doi]
AB  - The interplay between stem cells and their extracellular microenvironment is of 
      critical importance to the stem cell-based therapeutics in regenerative medicine. 
      Mineralized collagen is the main component of bone extracellular matrix, but the 
      effect of interfacial properties of mineralized collagen on subsequent cellular 
      behaviors is unclear. This study examined the role of surface chemistry of 
      nanoscale mineralized collagen on human periodontal ligament stem cell (hPDLSC) 
      fate decisions. The intrafibrillarly mineralized collagen (IMC), fabricated by a 
      biomimetic bottom-up approach, showed a bonelike hierarchy with 
      nanohydroxyapatites (HAs) periodically embedded within fibrils. The infrared 
      spectrum of the IMC showed the presence of phosphate, carbonate, amide I and II 
      bands; and infrared mapping displayed uniform and higher spatial distribution of 
      mineralization in the IMC. However, the distribution of the phosphate group 
      differed far from that of the amide I group in the extrafibrillarly mineralized 
      collagen (EMC), in which flowerlike HA clusters randomly depositing around the 
      surface of the fibrils. Moreover, a large quantity of extrafibrillar HAs covered 
      up the C horizontal lineO stretch and N-H in-plane bend, resulting in substantial reduction of 
      amide I and II bands. Cell experiments demonstrated that the hPDLSCs seeded on 
      the IMC exhibited a highly branched, osteoblast-like polygonal shape with 
      extended pseudopodia and thick stress fiber formation; while cells on the EMC 
      displayed a spindle shape with less branch points and thin actin fibril 
      formation. Furthermore, the biocompatibility of EMC was much lower than that of 
      IMC. Interestingly, even without osteogenic induction, mRNA levels of major 
      osteogenic differentiation genes were highly expressed in the IMC during 
      cultivation time. These data suggest that the IMC with a similar nanotopography 
      and surface chemistry to natural mineralized collagen directs hPDLSCs toward 
      osteoblast differentiation, providing a promising scaffold in bone tissue 
      regeneration.
FAU - Fu, Yu
AU  - Fu Y
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Liu, Shuai
AU  - Liu S
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Cui, Sheng-Jie
AU  - Cui SJ
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Kou, Xiao-Xing
AU  - Kou XX
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Wang, Xue-Dong
AU  - Wang XD
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Liu, Xiao-Mo
AU  - Liu XM
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Sun, Yue
AU  - Sun Y
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Wang, Gao-Nan
AU  - Wang GN
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Liu, Yan
AU  - Liu Y
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
FAU - Zhou, Yan-Heng
AU  - Zhou YH
AD  - Center for Craniofacial Stem Cell Research and Regeneration, Department of 
      Orthodontics, Peking University School and Hospital of Stomatology, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology , Beijing 100081, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20160617
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Differentiation/*drug effects
MH  - Collagen/*chemistry/*pharmacology
MH  - Humans
MH  - Osteogenesis
MH  - Periodontal Ligament/*cytology
MH  - Stem Cells/*cytology/drug effects
OTO - NOTNLM
OT  - cell fate
OT  - interfacial microenvironment
OT  - intrafibrillar mineralization
OT  - surface chemistry
OT  - tissue regeneration
EDAT- 2016/06/10 06:00
MHDA- 2018/09/12 06:00
CRDT- 2016/06/10 06:00
PHST- 2016/06/10 06:00 [entrez]
PHST- 2016/06/10 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
AID - 10.1021/acsami.6b04951 [pii]
AID - 10.1021/acsami.6b04951 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 Jun 29;8(25):15958-66. doi: 
      10.1021/acsami.6b04951. Epub 2016 Jun 17.