PMID- 27283677
OWN - NLM
STAT- MEDLINE
DCOM- 20170119
LR  - 20181202
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 211
DP  - 2016 Nov 15
TI  - RAPD and SCAR markers as potential tools for detection of milk origin in dairy 
      products: Adulterant sheep breeds in Serra da Estrela cheese production.
PG  - 631-6
LID - S0308-8146(16)30798-1 [pii]
LID - 10.1016/j.foodchem.2016.05.109 [doi]
AB  - Serra da Estrela Protected Designation of Origin (PDO) cheese is the most famous 
      Portuguese cheese and has a high commercial value. However, the adulteration of 
      production with cheaper/lower-quality milks from non-autochthones ovine breeds 
      compromises the quality of the final product and undervalues the original PDO 
      cheese. A Randomly Amplified Polymorphic DNA (RAPD) method was developed for 
      efficient detection of adulterant breeds in milk mixtures used for fraudulent 
      production of this cheese. Furthermore, Sequence Characterized Amplified Region 
      (SCAR) markers were designed envisioning the detection of milk adulteration in 
      processed dairy foods. The RAPD-SCAR technique is here described, for the first 
      time, to be potentially useful for detection of milk origin in dairy products. In 
      this sense, our findings will play an important role on the valorization of Serra 
      da Estrela cheese, as well as on other high-quality dairy products prone to 
      adulteration, contributing to the further development of the dairy industry.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Cunha, Joana T
AU  - Cunha JT
AD  - CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, 
      Portugal. Electronic address: jcunha@ceb.uminho.pt.
FAU - Ribeiro, Tania I B
AU  - Ribeiro TI
AD  - Center for Technology and Innovation, Agriculture and Food Technologies 
      Department, BLC3 Association - Platform for the Development of Center Inner 
      Region, Av. Calouste Gulbenkian n degrees  71 Zona Industrial, 3400-060 Oliveira do 
      Hospital, Portugal. Electronic address: tania.ribeiro@blc3.pt.
FAU - Rocha, Joao B
AU  - Rocha JB
AD  - Center for Technology and Innovation, Agriculture and Food Technologies 
      Department, BLC3 Association - Platform for the Development of Center Inner 
      Region, Av. Calouste Gulbenkian n degrees  71 Zona Industrial, 3400-060 Oliveira do 
      Hospital, Portugal. Electronic address: joao.bernardo@blc3.pt.
FAU - Nunes, Joao
AU  - Nunes J
AD  - Center for Technology and Innovation, Agriculture and Food Technologies 
      Department, BLC3 Association - Platform for the Development of Center Inner 
      Region, Av. Calouste Gulbenkian n degrees  71 Zona Industrial, 3400-060 Oliveira do 
      Hospital, Portugal. Electronic address: joao.nunes@blc3.pt.
FAU - Teixeira, Jose A
AU  - Teixeira JA
AD  - CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, 
      Portugal. Electronic address: jateixeira@deb.uminho.pt.
FAU - Domingues, Lucilia
AU  - Domingues L
AD  - CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, 
      Portugal. Electronic address: luciliad@deb.uminho.pt.
LA  - eng
PT  - Journal Article
DEP - 20160517
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
SB  - IM
MH  - Animals
MH  - Breeding/methods
MH  - Cheese/*analysis
MH  - Food Contamination/*analysis
MH  - Geography
MH  - Milk/*chemistry
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Portugal
MH  - Random Amplified Polymorphic DNA Technique/*methods
MH  - Sheep/*genetics
OTO - NOTNLM
OT  - Milk adulteration
OT  - Ovine breeds
OT  - Quality control
OT  - RAPD
OT  - SCAR
OT  - Serra da Estrela cheese
EDAT- 2016/06/11 06:00
MHDA- 2017/01/20 06:00
CRDT- 2016/06/11 06:00
PHST- 2015/11/20 00:00 [received]
PHST- 2016/05/10 00:00 [revised]
PHST- 2016/05/16 00:00 [accepted]
PHST- 2016/06/11 06:00 [entrez]
PHST- 2016/06/11 06:00 [pubmed]
PHST- 2017/01/20 06:00 [medline]
AID - S0308-8146(16)30798-1 [pii]
AID - 10.1016/j.foodchem.2016.05.109 [doi]
PST - ppublish
SO  - Food Chem. 2016 Nov 15;211:631-6. doi: 10.1016/j.foodchem.2016.05.109. Epub 2016 
      May 17.