PMID- 27284339
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 11
IP  - 6
DP  - 2016 Jun
TI  - MicroRNA-153 inhibits the proliferation and invasion of human laryngeal squamous 
      cell carcinoma by targeting KLF5.
PG  - 2503-2508
AB  - microRNA (miR)-153 has been shown to play a role in several solid malignancies; 
      however, its expression and function in laryngeal squamous cell carcinoma (LSCC) 
      have not been fully explored. In the present study, reverse 
      transcription-quantitative polymerase chain reaction analysis was performed in 
      order to detect the expression levels of miR-153 in mucosal specimens isolated 
      from patients undergoing total laryngectomy. In addition, in vitro experiments 
      were performed to analyze the cellular proliferation and invasion abilities of 
      human epithelial type 2 (HEp-2) cells transfected with miR-153 mimics or miR-153 
      antisense oligonucleotide (ASO). It was found that miR-153 was downregulated in 
      LSCC tissues. Furthermore, while miR-153 mimics inhibited cell proliferation and 
      invasion, miR-153 ASO promoted HEp-2 cell growth and invasion. At a molecular 
      level, it was predicted by bioinformatics that kruppel-like factor 5 (KLF5) has a 
      miR-153 binding site. Luciferase and protein expression analyses revealed that 
      miR-153 inhibited the protein expression of KLF5. These results suggest that 
      miR-153 may act as a tumor suppressor during LSCC progression via the suppression 
      of KLF5, and could potentially serve as a therapeutic target for LSCC.
FAU - Liu, Jian-Yong
AU  - Liu JY
AD  - Department of Otolaryngology, Zhangjiagang First People's Hospital, Suzhou, 
      Jiangsu 215600, P.R. China.
FAU - Lu, Jian-Bin
AU  - Lu JB
AD  - Department of Otolaryngology, Zhangjiagang First People's Hospital, Suzhou, 
      Jiangsu 215600, P.R. China.
FAU - Xu, Yue
AU  - Xu Y
AD  - Department of Otolaryngology, Zhangjiagang First People's Hospital, Suzhou, 
      Jiangsu 215600, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160324
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4887773
OTO - NOTNLM
OT  - kruppel-like factor 5
OT  - laryngeal squamous cell carcinoma
OT  - miR-153
OT  - microRNA
EDAT- 2016/06/11 06:00
MHDA- 2016/06/11 06:01
PMCR- 2016/03/24
CRDT- 2016/06/11 06:00
PHST- 2014/09/28 00:00 [received]
PHST- 2016/01/12 00:00 [accepted]
PHST- 2016/06/11 06:00 [entrez]
PHST- 2016/06/11 06:00 [pubmed]
PHST- 2016/06/11 06:01 [medline]
PHST- 2016/03/24 00:00 [pmc-release]
AID - ETM-0-0-3189 [pii]
AID - 10.3892/etm.2016.3189 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Jun;11(6):2503-2508. doi: 10.3892/etm.2016.3189. Epub 2016 Mar 
      24.