PMID- 27285735
OWN - NLM
STAT- MEDLINE
DCOM- 20170125
LR  - 20171116
IS  - 1477-2213 (Electronic)
IS  - 1028-6020 (Linking)
VI  - 18
IP  - 11
DP  - 2016 Nov
TI  - 12b-hydroxy-des-D-garcigerin A enhances glucose metabolism in insulin-resistant 
      HepG2 cells via the IRS-1/PI3-K/Akt cell signaling pathway.
PG  - 1091-100
LID - 10.1080/10286020.2016.1193489 [doi]
AB  - HepG2 cells were induced with a high concentration of insulin to establish an 
      insulin-resistant cell model (HepG2/IR). The effect of 
      12b-hydroxy-des-D-garcigerin A (DGA) on the glucose consumption (GC) of HepG2/IR 
      cells was analyzed with the glucose oxidase/peroxidase assay. The results showed 
      that DGA significantly stimulated GC by enhancing the activity of hexokinase (HK) 
      and pyruvate kinase (PK) in HepG2/IR cells. The cell signaling pathway by which 
      DGA enhances the GC of HepG2/IR cells was explored. The results showed that DGA 
      promoted the expression of insulin receptor (InsR) protein, and stimulated the 
      expression of insulin receptor substrate 1 (IRS-1), phosphatidylinositol-3 kinase 
      (p-PI3-K), and phospho-protein kinase B Serine(473) (p-AKT ser(473)). Therefore, 
      we concluded that DGA improved the insulin-resistance of HepG2/IR cells by 
      inducing the IRS-1/PI3-K/Akt cell signaling pathway. Interestingly, DGA had no 
      effect on the phosphorylation of threonine(172) (Thr(172)) in AMP-activated 
      protein kinase (AMPK).
FAU - Chen, Yu
AU  - Chen Y
AD  - a College of Chemistry and Material Sciences, South-Central University for 
      Nationalities , Wuhan 430074 , China.
FAU - Wang, Sha
AU  - Wang S
AD  - b College of Pharmacy, South-Central University for Nationalities , Wuhan 430074 
      , China.
FAU - Tian, Shi-Ting
AU  - Tian ST
AD  - c College of Life Science, South-Central University for Nationalities , Wuhan 
      430074 , China.
FAU - Hu, Xin
AU  - Hu X
AD  - b College of Pharmacy, South-Central University for Nationalities , Wuhan 430074 
      , China.
FAU - Xu, Jing
AU  - Xu J
AD  - b College of Pharmacy, South-Central University for Nationalities , Wuhan 430074 
      , China.
FAU - Yang, Guang-Zhong
AU  - Yang GZ
AD  - b College of Pharmacy, South-Central University for Nationalities , Wuhan 430074 
      , China.
FAU - Wang, Chao-Yuan
AU  - Wang CY
AD  - c College of Life Science, South-Central University for Nationalities , Wuhan 
      430074 , China.
LA  - eng
PT  - Journal Article
DEP - 20160610
PL  - England
TA  - J Asian Nat Prod Res
JT  - Journal of Asian natural products research
JID - 100888334
RN  - 0 (12b-hydroxy-des-D-garcigerin A)
RN  - 0 (Antifungal Agents)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Xanthones)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor, Insulin)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - Antifungal Agents/chemistry/isolation & purification/*pharmacology
MH  - Disease Models, Animal
MH  - Glucose/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Insulin Receptor Substrate Proteins
MH  - Insulin Resistance
MH  - Molecular Structure
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation/drug effects
MH  - Polymerase Chain Reaction
MH  - Receptor, Insulin/metabolism
MH  - Signal Transduction/drug effects
MH  - Xanthones/chemistry/isolation & purification/*pharmacology
OTO - NOTNLM
OT  - 12b-hydroxy-des-D-garcigerin A
OT  - AMP-activated protein kinase
OT  - HepG2
OT  - IRS-1/PI3-K/Akt cell signaling pathway
OT  - glucose consumption
OT  - insulin-resistance
EDAT- 2016/06/11 06:00
MHDA- 2017/01/26 06:00
CRDT- 2016/06/11 06:00
PHST- 2016/06/11 06:00 [entrez]
PHST- 2016/06/11 06:00 [pubmed]
PHST- 2017/01/26 06:00 [medline]
AID - 10.1080/10286020.2016.1193489 [doi]
PST - ppublish
SO  - J Asian Nat Prod Res. 2016 Nov;18(11):1091-100. doi: 
      10.1080/10286020.2016.1193489. Epub 2016 Jun 10.