PMID- 27285980
OWN - NLM
STAT- MEDLINE
DCOM- 20180213
LR  - 20201209
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 31
DP  - 2016 Aug 2
TI  - MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells 
      via down-regulated Mbnl3.
PG  - 48978-48994
LID - 10.18632/oncotarget.9832 [doi]
AB  - Neuraminidase (NA), a structural protein of the H9N2 avian influenza virus (H9N2 
      AIV), can facilitate viral invasion of the upper airway by cleaving the sialic 
      acid moieties on mucin. Dendritic cells (DCs) are major antigen-presenting cells 
      whose immune functions, such as presenting antigens and activating lymphocytes, 
      can be regulated by microRNAs. Here, we studied the molecular mechanism of 
      miRNA-induced repression of immune responses in mouse DCs. First, we screened for 
      and verified the miRNAs induced by NA. Then, we showed that, consistent with the 
      H9N2 virus treatment, the viral NA up-regulated the expression of miR-155, 
      miR-674, and miR-499 in DCs; however, unlike H9N2 virus treatment, the presence 
      of NA was associated with reduced expression of miR-181b1. Our results suggest 
      that NA significantly increased DC surface markers CD80 and MHCII and enhanced 
      the ability of activating lymphocytes and secreting cytokines compared with HA, 
      NP and M2. Meanwhile, we found that miR-674 and miR-155 over-expression increased 
      all surface markers of DC. Nevertheless, by inhibiting the expression of miR-674 
      and miR-155, NA lost the ability to promote DC maturation. Furthermore, we 
      predicted and demonstrated that Pgm2l1, Aldh18a1, Camk1d, and Mbnl3 were the 
      target genes of miR-674. Among them, Mbnl3 interference strongly blocked the 
      mature DCs. Collectively, our data shed new light on the roles of and mechanisms 
      involved in the repression of DCs by miRNAs, which may contribute to efforts to 
      develop a prophylaxis for the influenza virus.
FAU - Lin, Jian
AU  - Lin J
AD  - Life Science College, Nanjing Agricultural University, Weigang, Nanjing, Jiangsu, 
      PR China.
FAU - Chen, Ya T
AU  - Chen YT
AD  - Life Science College, Nanjing Agricultural University, Weigang, Nanjing, Jiangsu, 
      PR China.
FAU - Xia, Jing
AU  - Xia J
AD  - Life Science College, Nanjing Agricultural University, Weigang, Nanjing, Jiangsu, 
      PR China.
FAU - Yang, Qian
AU  - Yang Q
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Weigang, 
      Jiangsu, PR China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (MBNL3 protein, mouse)
RN  - 0 (MIRN674 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.2.1.18 (Neuraminidase)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/immunology
MH  - Carrier Proteins/*metabolism
MH  - Cell Proliferation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Down-Regulation
MH  - Gene Silencing
MH  - HEK293 Cells
MH  - Humans
MH  - Immune System
MH  - Influenza A Virus, H9N2 Subtype
MH  - Lentivirus
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*metabolism
MH  - Neuraminidase/*metabolism
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - RNA-Binding Proteins
PMC - PMC5226485
OTO - NOTNLM
OT  - Immune response
OT  - Immunity
OT  - Immunology and Microbiology Section
OT  - dendritic cells
OT  - immune regulation
OT  - miR-674
OT  - neuraminidase
OT  - virus replication
COIS- There is no conflict of interest.
EDAT- 2016/06/11 06:00
MHDA- 2018/02/14 06:00
PMCR- 2016/08/02
CRDT- 2016/06/11 06:00
PHST- 2015/11/01 00:00 [received]
PHST- 2016/05/02 00:00 [accepted]
PHST- 2016/06/11 06:00 [pubmed]
PHST- 2018/02/14 06:00 [medline]
PHST- 2016/06/11 06:00 [entrez]
PHST- 2016/08/02 00:00 [pmc-release]
AID - 9832 [pii]
AID - 10.18632/oncotarget.9832 [doi]
PST - ppublish
SO  - Oncotarget. 2016 Aug 2;7(31):48978-48994. doi: 10.18632/oncotarget.9832.