PMID- 27287433
OWN - NLM
STAT- MEDLINE
DCOM- 20170131
LR  - 20200325
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 161
IP  - 9
DP  - 2016 Sep
TI  - Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field 
      strains and attenuated strains of porcine epidemic diarrhea virus.
PG  - 2543-7
LID - 10.1007/s00705-016-2918-4 [doi]
AB  - Porcine epidemic diarrhea virus (PEDV) can cause serious disease and even death 
      in neonatal piglets, resulting in serious damage to the swine industry worldwide. 
      Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. 
      Previous studies have indicated that PEDV vaccine strains have a partial deletion 
      in ORF3. In this study, a nanoparticle-assisted polymerase chain reaction 
      (nanoparticle-assisted RT-PCR) assay targeting the ORF3 of PEDV was developed to 
      distinguish PEDV field strains from attenuated strains by using a specific pair 
      of primers. The PCR products of field strains and attenuated strains were 264 bp 
      and 215 bp in length, respectively. The sensitivity and specificity of this assay 
      were also assessed. The nanoparticle-assisted RT-PCR assay was 10-100 times more 
      sensitive than the conventional RT-PCR assay, with no cross-reactions when 
      amplifying porcine pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), 
      classical swine fever virus (CSFV), porcine parvovirus (PPV), porcine 
      reproductive and respiratory syndrome virus (PRRSV), porcine rotavirus (RV), and 
      porcine transmissible gastroenteritis virus (TGEV). The nanoparticle-assisted 
      RT-PCR assay we describe here can be used to distinguish field strains from 
      vaccine strains of PEDV, and it shows promise for reducing economic loss due to 
      PEDV infection.
FAU - Zhu, Yu
AU  - Zhu Y
AD  - Institute of Animal Science(IAS), Chinese Academy of Agricultural Sciences(CAAS), 
      Yuanmingyuan West Road 2, Hai Dian District, Beijing, 100193, China.
AD  - College of Animal Science and Technology, HLJ August First Land Reclamation 
      University, Daqing, 163319, China.
FAU - Wang, Gui-Hua
AU  - Wang GH
AD  - Animal Medical Center DBN Technology Group, Beijing, 100195, China.
FAU - Cui, Yu-Dong
AU  - Cui YD
AD  - College of Animal Science and Technology, HLJ August First Land Reclamation 
      University, Daqing, 163319, China. 1016856109@qq.com.
FAU - Cui, Shang-Jin
AU  - Cui SJ
AD  - Institute of Animal Science(IAS), Chinese Academy of Agricultural Sciences(CAAS), 
      Yuanmingyuan West Road 2, Hai Dian District, Beijing, 100193, China. 
      cuishangjin@caas.cn.
AD  - College of Animal Science and Technology, HLJ August First Land Reclamation 
      University, Daqing, 163319, China. cuishangjin@caas.cn.
LA  - eng
PT  - Journal Article
DEP - 20160610
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Coronavirus Infections/diagnosis/*veterinary/virology
MH  - Gene Expression Regulation, Viral
MH  - Mutation
MH  - *Nanoparticles
MH  - Porcine epidemic diarrhea virus/*classification/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Viral Proteins/genetics/metabolism
MH  - Viral Vaccines
PMC - PMC7087100
COIS- None of the authors of this paper has a financial or personal relationship with 
      other people or organizations that could inappropriately influence or bias the 
      content of the paper.
EDAT- 2016/06/12 06:00
MHDA- 2017/02/01 06:00
PMCR- 2020/03/23
CRDT- 2016/06/12 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/05/31 00:00 [accepted]
PHST- 2016/06/12 06:00 [entrez]
PHST- 2016/06/12 06:00 [pubmed]
PHST- 2017/02/01 06:00 [medline]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 10.1007/s00705-016-2918-4 [pii]
AID - 2918 [pii]
AID - 10.1007/s00705-016-2918-4 [doi]
PST - ppublish
SO  - Arch Virol. 2016 Sep;161(9):2543-7. doi: 10.1007/s00705-016-2918-4. Epub 2016 Jun 
      10.