PMID- 27288556 OWN - NLM STAT- MEDLINE DCOM- 20170929 LR - 20180430 IS - 1567-7257 (Electronic) IS - 1567-1348 (Linking) VI - 44 DP - 2016 Oct TI - Evaluation of infection status in Chinese swine with porcine reproductive and respiratory syndrome virus by nested RT-PCR targeting nsp2 gene. PG - 55-60 LID - S1567-1348(16)30244-1 [pii] LID - 10.1016/j.meegid.2016.06.020 [doi] AB - One of the biggest obstacles in containing porcine reproductive and respiratory syndrome virus (PRRSV) results from its genetic diversity due to the high mutation rate. The nsp2 gene of PRRSV is the most hypervariable region of the genome. Since the emergence of highly pathogenic (HP)-PRRSV, many of PRRSV strains with a mutated nsp2 gene have been reported. To decipher the epidemiology of the PRRSV and identify the epidemic strains, a nested RT-PCR able to differentiate the nsp2 gene from different PRRSV strains was developed and used to test 550 clinical samples. The amplified products of 301-bp, 211-bp and 154-bp were corresponding to low pathogenic PRRSV (LP-PRRSV) infection without deletion in nsp2 gene, HP-PRRSV infection with 90-bp deletion in nsp2 gene and a variant PRRSV strain with 147-bp deletion in nsp2 gene, respectively. Of the 550 clinical samples, 192 including 108 serum samples and 84 tissue samples were tested PRRSV RNA positive. Of the 192 positive samples, 107 were infected with a single strain and 85 were infected with two strains. 84 out of 85 samples harboring two PRRSV strains of HP-PRRSV and PRRSV variant strain were detected. 97 out of 107 samples with single strain were detected with HP-PRRSV infection. The data indicated that HP-PRRSV containing a 90-bp deletion in the nsp2 gene remained the predominant strain in China. CI - Copyright (c) 2016. Published by Elsevier B.V. FAU - Zhang, Minxia AU - Zhang M AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: 474659627@qq.com. FAU - Li, Xinan AU - Li X AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: xinan.1987@163.com. FAU - Cai, Xinna AU - Cai X AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: caixinna@126.com. FAU - Qu, Yajin AU - Qu Y AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: huiyaqui@163.com. FAU - Hu, Dongfang AU - Hu D AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: dongfang_hu@sina.com. FAU - Lv, Lin AU - Lv L AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: lvlin0911@163.com. FAU - Liu, Sidang AU - Liu S AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: liusid@sdau.edu.cn. FAU - Zhao, Xiaomin AU - Zhao X AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: xmzhao66@sdau.edu.cn. FAU - Wang, Leyi AU - Wang L AD - Animal Disease Diagnostic Laboratory, Ohio Department of Agriculture, Reynoldsburg, OH, USA. Electronic address: wang.1074@buckeyemail.osu.edu. FAU - Xiao, Yihong AU - Xiao Y AD - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, China. Electronic address: xiaoyihong01@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160608 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 RN - 0 (Viral Proteins) SB - IM MH - Animals MH - China MH - Porcine Reproductive and Respiratory Syndrome/*virology MH - Porcine respiratory and reproductive syndrome virus/*genetics/pathogenicity MH - Reproducibility of Results MH - Reverse Transcriptase Polymerase Chain Reaction/*methods MH - Sensitivity and Specificity MH - Swine MH - Viral Proteins/genetics OTO - NOTNLM OT - Co-infection OT - Deletion OT - Nested RT-PCR OT - Porcine reproductive and respiratory syndrome virus OT - nsp2 EDAT- 2016/06/12 06:00 MHDA- 2017/09/30 06:00 CRDT- 2016/06/12 06:00 PHST- 2016/03/02 00:00 [received] PHST- 2016/05/09 00:00 [revised] PHST- 2016/06/07 00:00 [accepted] PHST- 2016/06/12 06:00 [entrez] PHST- 2016/06/12 06:00 [pubmed] PHST- 2017/09/30 06:00 [medline] AID - S1567-1348(16)30244-1 [pii] AID - 10.1016/j.meegid.2016.06.020 [doi] PST - ppublish SO - Infect Genet Evol. 2016 Oct;44:55-60. doi: 10.1016/j.meegid.2016.06.020. Epub 2016 Jun 8.