PMID- 27288660
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20170313
IS  - 1873-2682 (Electronic)
IS  - 1011-1344 (Linking)
VI  - 161
DP  - 2016 Aug
TI  - Synthesis, characterization and anticancer activity studies of ruthenium(II) 
      polypyridyl complexes on A549 cells.
PG  - 295-303
LID - S1011-1344(16)30179-8 [pii]
LID - 10.1016/j.jphotobiol.2016.06.004 [doi]
AB  - Four new ruthenium(II) polypyridyl complexes [Ru(N-N)2(bddp)](ClO4)21-4 (N-N=dmb: 
      4,4'-dimethyl-2,2'-bipyridine 1, bpy: 2,2'-bipyridine 2, phen: 
      1,10-phenanthroline 3 and dmp: 2,9-dimethyl-1,10-phenanthroline 4, 
      bddp=benzilo[2,3-b]-1,4-diazabenzo[i]dipyrido[3,2-a:2',3'-c]phenazine) were 
      synthesized and characterized by elemental analysis, ESI-MS and (1)H NMR. The 
      cytotoxicity in vitro of the complexes against BEL-7402, HeLa, MG-63 and A549 
      cell lines was investigated by MTT method. The complexes show high cytotoxic 
      activity toward the selected cell lines with an IC50 value ranging from 5.3+/-0.6 
      to 15.7+/-3.6muM. The apoptosis was studied with acridine orange (AO)/ethdium 
      bromide (EB) and Hoechst 33258 staining methods. The cellular uptake was 
      investigated with DAPI staining method. The reactive oxygen species (ROS) and 
      mitochondrial membrane potential were performed under fluorescent microscope and 
      flow cytometry. The complexes can induce an increase in the ROS levels and a 
      decrease in the mitochondrial membrane potential. The comet assay was studied 
      with fluorescent microscope. The percentage in apoptotic and necrotic cells and 
      cell cycle arrest were assayed by flow cytometry. The effects of the complexes on 
      the expression of caspases and Bcl-2 family proteins were studied by western blot 
      analysis. The results show that the complexes induce apoptosis in A549 cells 
      through an ROS-mediated mitochondrial dysfunction pathway, which was accompanied 
      by regulating the expression of Bcl-2 family proteins.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Zeng, Chuan-Chuan
AU  - Zeng CC
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Jiang, Guang-Bin
AU  - Jiang GB
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Lai, Shang-Hai
AU  - Lai SH
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Zhang, Cheng
AU  - Zhang C
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Yin, Hui
AU  - Yin H
AD  - Department of Microbiology and Immunology, Guangdong Pharmaceutical University, 
      Guangzhou 510006, PR China. Electronic address: huiyin0103@163.com.
FAU - Tang, Bing
AU  - Tang B
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Wan, Dan
AU  - Wan D
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Liu, Yun-Jun
AU  - Liu YJ
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China. Electronic address: lyjche@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160605
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Coordination Complexes)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Reactive Oxygen Species)
RN  - 551W113ZEP (2,2'-Dipyridyl)
RN  - 7UI0TKC3U5 (Ruthenium)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - 2,2'-Dipyridyl/chemistry
MH  - A549 Cells
MH  - Antineoplastic Agents/*chemical synthesis/chemistry/toxicity
MH  - Apoptosis/drug effects
MH  - Caspases/metabolism
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Line, Tumor
MH  - Comet Assay
MH  - Coordination Complexes/*chemical synthesis/chemistry/toxicity
MH  - DNA Fragmentation/drug effects
MH  - HeLa Cells
MH  - Humans
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Microscopy, Fluorescence
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Ruthenium/*chemistry
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell cycle arrest
OT  - Cellular uptake
OT  - Ru(II) complex
OT  - Western blot analysis
EDAT- 2016/06/12 06:00
MHDA- 2017/03/14 06:00
CRDT- 2016/06/12 06:00
PHST- 2016/03/18 00:00 [received]
PHST- 2016/06/03 00:00 [revised]
PHST- 2016/06/03 00:00 [accepted]
PHST- 2016/06/12 06:00 [entrez]
PHST- 2016/06/12 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
AID - S1011-1344(16)30179-8 [pii]
AID - 10.1016/j.jphotobiol.2016.06.004 [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 2016 Aug;161:295-303. doi: 
      10.1016/j.jphotobiol.2016.06.004. Epub 2016 Jun 5.