PMID- 27289314
OWN - NLM
STAT- MEDLINE
DCOM- 20170222
LR  - 20220513
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 145
DP  - 2016 Sep 1
TI  - Biofunctionalization of titanium with bacitracin immobilization shows potential 
      for anti-bacteria, osteogenesis and reduction of macrophage inflammation.
PG  - 728-739
LID - S0927-7765(16)30425-8 [pii]
LID - 10.1016/j.colsurfb.2016.05.089 [doi]
AB  - Titanium has been widely used in the orthopedic and dental fields, however, the 
      inert nature of Ti makes it unsuitable for application in promoting bone cell 
      growth，osteogenic differentiation and antibacterial ability. The aims of the 
      current study were to investigate the antimicrobial activity and biofunction of 
      the polypeptide antibiotic bacitracin, and obtain a multi-biofunctional titanium 
      implant by covalently-immobilizing titanium with the bacitracin. The results 
      showed that the bacitracin possessed low minimum inhibitory concentration (MIC) 
      to both Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus 
      (MRSA), with the non-cytotoxicity concentration up to 500mug/mL to human bone 
      marrow mesenchymal stem cells (hBMSCs), furthermore, the bacitracin could improve 
      the osteogenic differentiation of hBMSCs. The results of Scanning electron 
      microscope (SEM) and X-ray photoelectron spectroscopy (XPS) indicated that 
      bacitracin had been covalently immobilized on the surface of titanium. 
      Immobilized bacitracin could improve the hydrophilic of immobilized titanium. The 
      results of antimicrobial assay demonstrated that the covalently-immobilized 
      bacitracin also had excellent antimicrobial property, and the bacitracin 
      immobilized titanium could inhibit bacterial adhesion and colonization. The 
      results of cell biology experiments proved that the bacitracin immobilized 
      titanium could improve hBMSCs' adhesion, proliferation and osteogenic 
      differentiation. We also found that the macrophages were difficult to spread or 
      activate on the surface of bacitracin immobilized titanium, and the secretion of 
      inflammatory factors had been inhibited. In conclusion, the novel bacitracin 
      immobilized titanium has multi-biofunctions including outstanding antibacterial 
      properties, excellent cell biology performance, and restraining inflammation, 
      which has exciting application prospect.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Nie, Bin'en
AU  - Nie B
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, PR China.
FAU - Ao, Haiyong
AU  - Ao H
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, PR China.
FAU - Zhou, Jianliang
AU  - Zhou J
AD  - Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang 
      University, PR China.
FAU - Tang, Tingting
AU  - Tang T
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, PR China.
FAU - Yue, Bing
AU  - Yue B
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, PR China. Electronic address: advbmp2@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160531
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 1405-87-4 (Bacitracin)
RN  - D1JT611TNE (Titanium)
SB  - IM
EIN - Colloids Surf B Biointerfaces. 2022 Aug;216:112550. PMID: 35561633
MH  - Bacitracin/adverse effects/*chemistry/*pharmacology
MH  - Bone Marrow Cells/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Macrophages/drug effects/*metabolism
MH  - Mesenchymal Stem Cells/cytology/drug effects
MH  - Methicillin-Resistant Staphylococcus aureus/drug effects
MH  - Microscopy, Electrochemical, Scanning
MH  - Osteogenesis/drug effects
MH  - Photoelectron Spectroscopy
MH  - Staphylococcus aureus/drug effects
MH  - Titanium/*chemistry
OTO - NOTNLM
OT  - Anti-bacteria
OT  - Bacitracin
OT  - Biofunctionalization
OT  - Macrophage inflammation
OT  - Osteogenesis
OT  - Titanium
EDAT- 2016/06/13 06:00
MHDA- 2017/02/23 06:00
CRDT- 2016/06/13 06:00
PHST- 2016/01/14 00:00 [received]
PHST- 2016/05/10 00:00 [revised]
PHST- 2016/05/28 00:00 [accepted]
PHST- 2016/06/13 06:00 [entrez]
PHST- 2016/06/13 06:00 [pubmed]
PHST- 2017/02/23 06:00 [medline]
AID - S0927-7765(16)30425-8 [pii]
AID - 10.1016/j.colsurfb.2016.05.089 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2016 Sep 1;145:728-739. doi: 
      10.1016/j.colsurfb.2016.05.089. Epub 2016 May 31.