PMID- 27290728
OWN - NLM
STAT- MEDLINE
DCOM- 20170410
LR  - 20220408
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 244
IP  - 4
DP  - 2016 Oct
TI  - New molecular markers and cytogenetic probes enable chromosome identification of 
      wheat-Thinopyrum intermedium introgression lines for improving protein and gluten 
      contents.
PG  - 865-76
LID - 10.1007/s00425-016-2554-y [doi]
AB  - New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 
      chromosome, and novel FISH probe representing the terminal repeats was produced 
      for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been 
      used as a valuable resource for improving the disease resistance and yield 
      potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 
      substitution and translocation has displayed superior grain protein and wet 
      gluten content. With the aim to develop a number of chromosome 1St#2 specific 
      molecular and cytogenetic markers, a high throughput, low-cost specific-locus 
      amplified fragment sequencing (SLAF-seq) technology was used to compare the 
      sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related 
      species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic 
      fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. 
      Thirty-seven specific molecular markers were validated by PCR from 50 randomly 
      selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the 
      family level between wheat and St genomes was compared using the SLAFs. A new 
      oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for 
      fluorescence in situ hybridization (FISH), and was observed to hybridize to the 
      terminal region of 1St#L and also onto the terminal heterochromatic region of Th. 
      intermedium genomes. The genome-wide markers and repetitive based probe 
      Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in 
      wheat backgrounds.
FAU - Li, Guangrong
AU  - Li G
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, Sichuan, China.
FAU - Wang, Hongjin
AU  - Wang H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, Sichuan, China.
FAU - Lang, Tao
AU  - Lang T
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, Sichuan, China.
FAU - Li, Jianbo
AU  - Li J
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, Sichuan, China.
FAU - La, Shixiao
AU  - La S
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, Sichuan, China.
FAU - Yang, Ennian
AU  - Yang E
AD  - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 
      610066, Sichuan, China.
FAU - Yang, Zujun
AU  - Yang Z
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, Sichuan, China. yangzujun@uestc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160611
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (DNA Probes)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Genetic Markers)
RN  - 0 (Plant Proteins)
RN  - 8002-80-0 (Glutens)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - DNA Probes/genetics
MH  - DNA Transposable Elements/genetics
MH  - Genetic Markers/genetics
MH  - Genome, Plant/genetics
MH  - Glutens/*metabolism
MH  - High-Throughput Nucleotide Sequencing
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding/methods
MH  - Plant Proteins/*metabolism
MH  - Poaceae/*genetics/metabolism
MH  - Species Specificity
MH  - Triticum/*genetics/metabolism
OTO - NOTNLM
OT  - Grain quality
OT  - In situ hybridization
OT  - Molecular markers
OT  - Thinopyrum intermedium
OT  - Wheat
EDAT- 2016/06/13 06:00
MHDA- 2017/04/11 06:00
CRDT- 2016/06/13 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2016/06/06 00:00 [accepted]
PHST- 2016/06/13 06:00 [entrez]
PHST- 2016/06/13 06:00 [pubmed]
PHST- 2017/04/11 06:00 [medline]
AID - 10.1007/s00425-016-2554-y [pii]
AID - 10.1007/s00425-016-2554-y [doi]
PST - ppublish
SO  - Planta. 2016 Oct;244(4):865-76. doi: 10.1007/s00425-016-2554-y. Epub 2016 Jun 11.