PMID- 27291351
OWN - NLM
STAT- MEDLINE
DCOM- 20170301
LR  - 20170302
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 55
DP  - 2016 Aug
TI  - Response of selenium-dependent glutathione peroxidase in the freshwater bivalve 
      Anodonta woodiana exposed to 2,4-dichlorophenol,2,4,6-trichlorophenol and 
      pentachlorophenol.
PG  - 499-509
LID - S1050-4648(16)30372-2 [pii]
LID - 10.1016/j.fsi.2016.06.012 [doi]
AB  - 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP), and 
      pentachlorophenol (PCP) pose a health risk to aquatic organism and humans, and 
      are recognized as persistent priority pollutants. Selenium dependent glutathione 
      peroxidase (Se-GPx) belongs to the family of selenoprotein, which acts mainly as 
      an antioxidant role in the cellular defense system. In the current study, a 
      Se-GPx full length cDNA was cloned from Anodonta woodiana and named as AwSeGPx. 
      It had a characteristic codon at 165TGA167 that corresponds to 
      selenocysteine(Sec) amino acid as U44. The full length cDNA consists of 870 bp, 
      an open reading frame (ORF) of 585 bp encoded a polypeptide of 195 amino in which 
      conserved domain (68LGFPCNQF75) and a glutathione peroxide-1 GPx active site 
      (32GKVILVENVASLUGTT47) were observed. Additionally, the eukaryotic selenocysteine 
      insertion sequence (SECIS) was conserved in the 3'UTR. The AwSeGPx amino acid 
      sequence exhibited a high similarity with that of other Se-GPx. Real-time PCR 
      analysis revealed that AwSeGPx mRNA had a widely distribution, but the highest 
      level was observed in hepatopancreas. AwSeGPx mRNA expression was significantly 
      up-regulated in hepatopancreas, gill and hemocytes after 2,4-DCP, 2,4,6-TCP and 
      PCP exposure. Under similar environment, clams A. woodiana showed a more 
      sensitive to PCP than that of 2,4-DCP and 2,4,6-TCP. These results indicate that 
      AwSeGPx plays a protective role in eliminating oxidative stress derived from 
      2,4-DCP, 2,4,6-TCP and PCP treatment.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Xia, Xichao
AU  - Xia X
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China; State Key Laboratory of Environmental Chemistry and 
      Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy 
      of Sciences, Beijing 100085, China. Electronic address: xiaxichao8336@163.com.
FAU - Hua, Chunxiu
AU  - Hua C
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China.
FAU - Xue, Shipeng
AU  - Xue S
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China.
FAU - Shi, Bingqin
AU  - Shi B
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China.
FAU - Gui, Gaixia
AU  - Gui G
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China.
FAU - Zhang, Dongxian
AU  - Zhang D
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China.
FAU - Wang, Xiying
AU  - Wang X
AD  - Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan 
      Province, China.
FAU - Guo, Lianghong
AU  - Guo L
AD  - State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research 
      Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China. Electronic address: lhguo@rcees.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20160609
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Chlorophenols)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - D9BSU0SE4T (Pentachlorophenol)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anodonta/*drug effects/*genetics/metabolism
MH  - Base Sequence
MH  - Chlorophenols/toxicity
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/metabolism
MH  - Glutathione Peroxidase/chemistry/*genetics/metabolism
MH  - Pentachlorophenol/toxicity
MH  - Phylogeny
MH  - Protein Conformation
MH  - Protein Structure, Secondary
MH  - RNA, Messenger/genetics/metabolism
MH  - Random Allocation
MH  - Sequence Alignment
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - 2,4,6-Trichlorophenol
OT  - 2,4-Dichlorophenol
OT  - Anodonta woodiana
OT  - AwSeGPx
OT  - Pentachlorophenol
EDAT- 2016/06/14 06:00
MHDA- 2017/03/03 06:00
CRDT- 2016/06/14 06:00
PHST- 2016/03/10 00:00 [received]
PHST- 2016/06/08 00:00 [revised]
PHST- 2016/06/08 00:00 [accepted]
PHST- 2016/06/14 06:00 [entrez]
PHST- 2016/06/14 06:00 [pubmed]
PHST- 2017/03/03 06:00 [medline]
AID - S1050-4648(16)30372-2 [pii]
AID - 10.1016/j.fsi.2016.06.012 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Aug;55:499-509. doi: 10.1016/j.fsi.2016.06.012. Epub 
      2016 Jun 9.