PMID- 27291656
OWN - NLM
STAT- MEDLINE
DCOM- 20170829
LR  - 20180518
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 16
IP  - 17
DP  - 2016 Sep
TI  - Interactome analysis of the EV71 5' untranslated region in differentiated 
      neuronal cells SH-SY5Y and regulatory role of FBP3 in viral replication.
PG  - 2351-62
LID - 10.1002/pmic.201600098 [doi]
AB  - Enterovirus 71 (EV71), a single-stranded RNA virus, is one of the most serious 
      neurotropic pathogens in the Asia-Pacific region. Through interactions with host 
      proteins, the 5' untranslated region (5'UTR) of EV71 is important for viral 
      replication. To gain a protein profile that interact with the EV71 5'UTR in 
      neuronal cells, we performed a biotinylated RNA-protein pull-down assay in 
      conjunction with LC-MS/MS analysis. A total of 109 proteins were detected and 
      subjected to Database for Annotation, Visualization and Integrated Discovery 
      (DAVID) analyses. These proteins were found to be highly correlated with 
      biological processes including RNA processing/splicing, epidermal cell 
      differentiation, and protein folding. A protein-protein interaction network was 
      constructed using the STRING online database to illustrate the interactions of 
      those proteins that are mainly involved in RNA processing/splicing or protein 
      folding. Moreover, we confirmed that the far-upstream element binding protein 3 
      (FBP3) was able to bind to the EV71 5'UTR. The redistribution of FBP3 in 
      subcellular compartments was observed after EV71 infection, and the decreased 
      expression of FBP3 in host neuronal cells markedly inhibited viral replication. 
      Our results reveal various host proteins that potentially interact with the EV71 
      5'UTR in neuronal cells, and we found that FBP3 could serve as a positive 
      regulator in host cells.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Huang, Hsing-I
AU  - Huang HI
AD  - Research Center for Emerging Viral Infections, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taiwan.
FAU - Chang, Ying-Ying
AU  - Chang YY
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Lin, Jhao-Yin
AU  - Lin JY
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Kuo, Rei-Lin
AU  - Kuo RL
AD  - Research Center for Emerging Viral Infections, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taiwan.
FAU - Liu, Hao-Ping
AU  - Liu HP
AD  - Department of Veterinary Medicine, National Chung Hsing University, Taichung, 
      Taiwan.
FAU - Shih, Shin-Ru
AU  - Shih SR
AD  - Research Center for Emerging Viral Infections, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Clinical Virology Lab, Department of Medical Technology, Chang Gung Memorial 
      Hospital, Linkou, Taiwan.
FAU - Wu, Chih-Ching
AU  - Wu CC
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan. luckywu@mail.cgu.edu.tw.
AD  - Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung 
      University, Kwei-Shan, Tao-Yuan, Taiwan. luckywu@mail.cgu.edu.tw.
AD  - Molecular Medicine Research Center, Chang Gung University, Kwei-Shan, Tao-Yuan, 
      Taiwan. luckywu@mail.cgu.edu.tw.
AD  - Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, 
      Linkou, Taiwan. luckywu@mail.cgu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160708
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FUBP3 protein, human)
RN  - 0 (RNA, Viral)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Cell Line
MH  - DNA-Binding Proteins/*metabolism
MH  - Enterovirus A, Human/genetics/*physiology
MH  - Enterovirus Infections/*metabolism/pathology
MH  - Humans
MH  - Neurons/metabolism/pathology/*virology
MH  - RNA, Viral/genetics/*metabolism
MH  - Transcription Factors/*metabolism
MH  - *Virus Replication
OTO - NOTNLM
OT  - Differentiated neuronal cells
OT  - EV71
OT  - FBP3
OT  - Internal ribosomal entry site
OT  - Microbiology
EDAT- 2016/06/14 06:00
MHDA- 2017/08/30 06:00
CRDT- 2016/06/14 06:00
PHST- 2016/02/14 00:00 [received]
PHST- 2016/04/13 00:00 [revised]
PHST- 2016/06/08 00:00 [accepted]
PHST- 2016/06/14 06:00 [entrez]
PHST- 2016/06/14 06:00 [pubmed]
PHST- 2017/08/30 06:00 [medline]
AID - 10.1002/pmic.201600098 [doi]
PST - ppublish
SO  - Proteomics. 2016 Sep;16(17):2351-62. doi: 10.1002/pmic.201600098. Epub 2016 Jul 
      8.