PMID- 27294001
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160613
LR  - 20201001
IS  - 2156-6976 (Print)
IS  - 2156-6976 (Electronic)
IS  - 2156-6976 (Linking)
VI  - 6
IP  - 5
DP  - 2016
TI  - Decreased 5-Hydroxymethylcytosine (5-hmC) predicts poor prognosis in early-stage 
      laryngeal squamous cell carcinoma.
PG  - 1089-98
AB  - Accumulating evidences suggest that large-scale loss of 5-hydroxymethylcytosine 
      (5-hmC) is an epigenetic hallmark in different cancers. However, the levels of 
      5-hmC in laryngeal squamous cell carcinoma (LSCC) and its prognostic value in 
      this cancer remain largely unknown. Using dot-blot and quantitative RT-PCR 
      assays, we investigate 5-hmC levels and expression of TET-1, -2 and -3 in LSCCs 
      and explore the association of 5-hmC levels with clinicopathological 
      characteristics and clinical outcome of LSCC patients. Our data showed that 5-hmC 
      was significantly decreased in LSCCs as compared with matched normal tissues. We 
      also found a strong link between decreased 5-hmC and the reduction of TET-1 gene 
      expression, but not TET-2 or -3, suggesting that decreased TET-1 expression was 
      implicated in 5-hmC loss in LSCC. Moreover, Mann-Whitney U tests showed that 
      5-hmC content was significantly associated with smoking (P = 0.039) and tumor 
      invasion (P = 0.004). Importantly, we found that decreased 5-hmC was 
      significantly associated with poor survival of early-stage LSCC patients (P = 
      0.043). Altogether, our findings implicate that decreased 5-hmC probaly caused by 
      the reduction of TET-1 is crucial to the clinical pathology of LSCC and is a poor 
      prognostic factor in ealry-stage LSCC patients.
FAU - Zhang, Yanfang
AU  - Zhang Y
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong 
      UniversityXi'an 710061, The People's Republic of China; Department of 
      Endocrinology, Luoyang Central Hospital Affiliated to Zhengzhou UniversityLuoyang 
      471009, The People's Republic of China.
FAU - Wu, Kexia
AU  - Wu K
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710061, The People's Republic of China.
FAU - Shao, Yuan
AU  - Shao Y
AD  - Department of Otolaryngology, The First Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710061, The People's Republic of China.
FAU - Sui, Fang
AU  - Sui F
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710061, The People's Republic of China.
FAU - Yang, Qi
AU  - Yang Q
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710061, The People's Republic of China.
FAU - Shi, Bingyin
AU  - Shi B
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710061, The People's Republic of China.
FAU - Hou, Peng
AU  - Hou P
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710061, The People's Republic of China.
FAU - Ji, Meiju
AU  - Ji M
AD  - Center for Translational Medicine, The First Affiliated Hospital of Xi'an 
      Jiaotong University Xi'an 710061, The People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20160501
PL  - United States
TA  - Am J Cancer Res
JT  - American journal of cancer research
JID - 101549944
PMC - PMC4889722
OTO - NOTNLM
OT  - 5-hydroxymethylcytosine (5-hmC)
OT  - Laryngeal squamous cell carcinoma (LSCC)
OT  - TET-1
OT  - poor survival
EDAT- 2016/06/14 06:00
MHDA- 2016/06/14 06:01
PMCR- 2016/05/01
CRDT- 2016/06/14 06:00
PHST- 2016/04/14 00:00 [received]
PHST- 2016/04/18 00:00 [accepted]
PHST- 2016/06/14 06:00 [entrez]
PHST- 2016/06/14 06:00 [pubmed]
PHST- 2016/06/14 06:01 [medline]
PHST- 2016/05/01 00:00 [pmc-release]
PST - epublish
SO  - Am J Cancer Res. 2016 May 1;6(5):1089-98. eCollection 2016.