PMID- 27294276
OWN - NLM
STAT- MEDLINE
DCOM- 20170324
LR  - 20181113
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 39
IP  - 4
DP  - 2016 Aug
TI  - Coptisine Prevented IL-beta-Induced Expression of Inflammatory Mediators in 
      Chondrocytes.
PG  - 1558-65
LID - 10.1007/s10753-016-0391-6 [doi]
AB  - Interleukin 1beta (IL-1beta) is a pleiotropic pro-inflammatory cytokine that plays a 
      critical role in the development of osteoarthritis (OA). Coptisine is an 
      isoquinoline alkaloid extracted from Coptidis rhizome and has been reported to 
      possess anti-inflammatory activity. However, the anti-inflammatory effects of 
      coptisine on interleukin-1 beta (IL-1beta)-stimulated chondrocytes have not been 
      reported. Therefore, the aim of this study was to investigate the effects of 
      coptisine on IL-1beta-induced inflammation in human articular chondrocytes. Our 
      results showed that coptisine greatly inhibited the production of nitric oxide 
      (NO) and prostaglandin E2 (PGE2), as well as suppressed the expression of 
      inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in human OA 
      chondrocytes induced by IL-1beta. It also inhibited the expression of matrix 
      metalloproteinase-3 (MMP-3) and MMP-13 in IL-1beta-stimulated human OA chondrocytes. 
      Furthermore, coptisine significantly inhibited the IL-1beta-induced NF-kB activation 
      in human OA chondrocytes. Taken together, these data suggest that coptisine 
      inhibits the IL-1beta-induced inflammatory response by suppressing the NF-kB 
      signaling pathway. Thus, coptisine may be a potential agent in the treatment of 
      OA.
FAU - Zhou, Kai
AU  - Zhou K
AD  - Department of Emergency, The Affiliated Hospital of Southwest Medical University, 
      No. 25 Taiping Road, Luzhou, 646000, China. zhoukailuz@163.com.
FAU - Hu, Li
AU  - Hu L
AD  - Department of Emergency, The Affiliated Hospital of Southwest Medical University, 
      No. 25 Taiping Road, Luzhou, 646000, China.
FAU - Liao, Wenjun
AU  - Liao W
AD  - Department of Emergency, The Affiliated Hospital of Southwest Medical University, 
      No. 25 Taiping Road, Luzhou, 646000, China.
FAU - Yin, Defeng
AU  - Yin D
AD  - Department of Emergency, The Affiliated Hospital of Southwest Medical University, 
      No. 25 Taiping Road, Luzhou, 646000, China.
FAU - Rui, Feng
AU  - Rui F
AD  - Basic Medical College of Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, 
      830054, China. ruifang_phd@163.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - 0GCL71VN14 (coptisine)
RN  - 0I8Y3P32UF (Berberine)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Berberine/*analogs & derivatives/pharmacology
MH  - Cells, Cultured
MH  - Chondrocytes/*metabolism/pathology
MH  - Cyclooxygenase 2/drug effects
MH  - Humans
MH  - Inflammation Mediators/*antagonists & inhibitors/metabolism
MH  - Interleukin-1beta/*physiology
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide Synthase Type II/antagonists & inhibitors
OTO - NOTNLM
OT  - chondrocyte
OT  - coptisine
OT  - interleukin-1 beta (IL-1beta)
OT  - osteoarthritis (OA)
EDAT- 2016/06/14 06:00
MHDA- 2017/03/25 06:00
CRDT- 2016/06/14 06:00
PHST- 2016/06/14 06:00 [entrez]
PHST- 2016/06/14 06:00 [pubmed]
PHST- 2017/03/25 06:00 [medline]
AID - 10.1007/s10753-016-0391-6 [pii]
AID - 10.1007/s10753-016-0391-6 [doi]
PST - ppublish
SO  - Inflammation. 2016 Aug;39(4):1558-65. doi: 10.1007/s10753-016-0391-6.