PMID- 27294852
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20201209
IS  - 2150-5608 (Electronic)
IS  - 2150-5594 (Print)
IS  - 2150-5594 (Linking)
VI  - 8
IP  - 1
DP  - 2017 Jan 2
TI  - Dectin-1 is required for miR155 upregulation in murine macrophages in response to 
      Candida albicans.
PG  - 41-52
LID - 10.1080/21505594.2016.1200215 [doi]
AB  - The commensal fungal pathogen Candida albicans is a leading cause of lethal 
      systemic infections in immunocompromised patients. One of the main mechanisms of 
      host immune evasion and virulence by this pathogen is the switch from yeast form 
      to hyphal growth morphologies. Micro RNAs (miRNAs), a small regulatory non-coding 
      RNA, has been identified as an important part of the immune response to a wide 
      variety of pathogens. In general, miRNAs act by modulating the intensity of 
      inflammatory responses. miRNAs act by base-paring binding to specific sequences 
      of target mRNAs, generally causing their silencing through mRNA degradation or 
      translational repression. To study the impact of C. albicans cell morphology upon 
      host miRNA expression, we investigated the differential modulation of 9 different 
      immune response-related miRNAs in primary murine bone marrow-derived macrophages 
      (BMDMs) exposed to either yeasts or hyphal forms of Candida albicans. Here, we 
      show that the different growth morphologies induce distinct miRNA expression 
      patterns in BMDMs. Interestingly, our data suggest that the C-Type lectin 
      receptor Dectin-1 is a major PRR that orchestrates miR155 upregulation in a 
      Syk-dependent manner. Our results suggest that PRR-mediating signaling events are 
      key drivers of miRNA-mediated gene regulation during fungal pathogenesis.
FAU - Agustinho, Daniel Paiva
AU  - Agustinho DP
AD  - a Departamento de Biologia Celular , Laboratorio de Biologia Molecular, 
      Universidade de Brasilia , Brasilia , DF , Brasil.
FAU - de Oliveira, Marco Antonio
AU  - de Oliveira MA
AD  - a Departamento de Biologia Celular , Laboratorio de Biologia Molecular, 
      Universidade de Brasilia , Brasilia , DF , Brasil.
FAU - Tavares, Aldo Henrique
AU  - Tavares AH
AD  - b Departamento de Biologia Celular , Laboratorio de Imunologia Aplicada, 
      Instituto de Biologia, Universidade de Brasilia , Brasilia , DF , Brasil.
FAU - Derengowski, Lorena
AU  - Derengowski L
AD  - a Departamento de Biologia Celular , Laboratorio de Biologia Molecular, 
      Universidade de Brasilia , Brasilia , DF , Brasil.
FAU - Stolz, Valentina
AU  - Stolz V
AD  - c Department of Molecular Genetics , Max F. Perutz Laboratories, Medical 
      University of Vienna , Vienna , Austria.
FAU - Guilhelmelli, Fernanda
AU  - Guilhelmelli F
AD  - a Departamento de Biologia Celular , Laboratorio de Biologia Molecular, 
      Universidade de Brasilia , Brasilia , DF , Brasil.
FAU - Mortari, Marcia Renata
AU  - Mortari MR
AD  - d Departamento de Ciencias Fisiologicas , Laboratorio de Neurofarmacologia, 
      Universidade de Brasilia , Brasilia , DF , Brasil.
FAU - Kuchler, Karl
AU  - Kuchler K
AD  - c Department of Molecular Genetics , Max F. Perutz Laboratories, Medical 
      University of Vienna , Vienna , Austria.
FAU - Silva-Pereira, Ildinete
AU  - Silva-Pereira I
AD  - a Departamento de Biologia Celular , Laboratorio de Biologia Molecular, 
      Universidade de Brasilia , Brasilia , DF , Brasil.
LA  - eng
PT  - Journal Article
DEP - 20160613
PL  - United States
TA  - Virulence
JT  - Virulence
JID - 101531386
RN  - 0 (Lectins, C-Type)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (Receptors, Pattern Recognition)
RN  - 0 (dectin 1)
RN  - EC 2.7.10.2 (Syk Kinase)
SB  - IM
CIN - Virulence. 2017 Jan 2;8(1):8-10. PMID: 27441402
MH  - Animals
MH  - Candida albicans/*cytology/growth & development/immunology/*pathogenicity
MH  - Gene Expression Regulation, Fungal
MH  - Hyphae/immunology/pathogenicity/physiology
MH  - Immune Evasion
MH  - Lectins, C-Type/genetics/*metabolism
MH  - Macrophages/immunology/*microbiology
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Receptors, Pattern Recognition/metabolism
MH  - Signal Transduction
MH  - Syk Kinase/metabolism
MH  - Transcriptional Activation
MH  - Up-Regulation
PMC - PMC5963198
OTO - NOTNLM
OT  - Candida albicans
OT  - immune response
OT  - macrophages
OT  - miR155
OT  - miRNA
EDAT- 2016/06/14 06:00
MHDA- 2017/07/27 06:00
PMCR- 2017/06/13
CRDT- 2016/06/14 06:00
PHST- 2016/06/14 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/06/14 06:00 [entrez]
PHST- 2017/06/13 00:00 [pmc-release]
AID - 1200215 [pii]
AID - 10.1080/21505594.2016.1200215 [doi]
PST - ppublish
SO  - Virulence. 2017 Jan 2;8(1):41-52. doi: 10.1080/21505594.2016.1200215. Epub 2016 
      Jun 13.