PMID- 27298519
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20181113
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2016
DP  - 2016
TI  - Autocrine Acetylcholine, Induced by IL-17A via NFkappaB and ERK1/2 Pathway 
      Activation, Promotes MUC5AC and IL-8 Synthesis in Bronchial Epithelial Cells.
PG  - 9063842
LID - 10.1155/2016/9063842 [doi]
LID - 9063842
AB  - IL-17A is overexpressed in the lung during acute neutrophilic inflammation. 
      Acetylcholine (ACh) increases IL-8 and Muc5AC production in airway epithelial 
      cells. We aimed to characterize the involvement of nonneuronal components of 
      cholinergic system on IL-8 and Muc5AC production in bronchial epithelial cells 
      stimulated with IL-17A. Bronchial epithelial cells were stimulated with 
      recombinant human IL-17A (rhIL-17A) to evaluate the ChAT expression, the ACh 
      binding and production, the IL-8 release, and the Muc5AC production. Furthermore, 
      the effectiveness of PD098,059 (inhibitor of MAPKK activation), Bay11-7082 
      (inhibitor of IkBalpha phosphorylation), Hemicholinium-3 (HCh-3) (choline uptake 
      blocker), and Tiotropium bromide (Spiriva(R)) (anticholinergic drug) was tested in 
      our in vitro model. We showed that rhIL-17A increased the expression of ChAT, the 
      levels of ACh binding and production, and the IL-8 and Muc5AC production in 
      stimulated bronchial epithelial cells compared with untreated cells. The 
      pretreatment of the cells with PD098,059 and Bay11-7082 decreased the ChAT 
      expression and the ACh production/binding, while HCh-3 and Tiotropium decreased 
      the IL-8 and Muc5AC synthesis in bronchial epithelial cells stimulated with 
      rhIL-17A. IL-17A is involved in the IL-8 and Muc5AC production promoting, via 
      NFkappaB and ERK1/2 pathway activation, the synthesis of ChAT, and the related 
      activity of autocrine ACh in bronchial epithelial cells.
FAU - Montalbano, Angela Marina
AU  - Montalbano AM
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Albano, Giusy Daniela
AU  - Albano GD
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Bonanno, Anna
AU  - Bonanno A
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Riccobono, Loredana
AU  - Riccobono L
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Di Sano, Caterina
AU  - Di Sano C
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Ferraro, Maria
AU  - Ferraro M
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Siena, Liboria
AU  - Siena L
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Anzalone, Giulia
AU  - Anzalone G
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy; Dipartimento di 
      Biomedicina Sperimentale e Neuroscienze Cliniche (BioNec), 90127 Palermo, Italy.
FAU - Gagliardo, Rosalia
AU  - Gagliardo R
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Pieper, Michael Paul
AU  - Pieper MP
AD  - Boehringer Ingelheim Pharma GmbH & Co. KG, 88400 Biberach, Germany.
FAU - Gjomarkaj, Mark
AU  - Gjomarkaj M
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
FAU - Profita, Mirella
AU  - Profita M
AD  - Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National 
      Research Council of Italy (CNR), 90146 Palermo, Italy.
LA  - eng
PT  - Journal Article
DEP - 20160519
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-8)
RN  - 0 (MUC5AC protein, human)
RN  - 0 (Mucin 5AC)
RN  - 0 (NF-kappa B)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/*metabolism
MH  - Autocrine Communication/drug effects
MH  - Bronchi/cytology
MH  - Cell Line
MH  - Epithelial Cells/*drug effects/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-17/*pharmacology
MH  - Interleukin-8/*metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Mucin 5AC/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC4889862
EDAT- 2016/06/15 06:00
MHDA- 2017/05/16 06:00
PMCR- 2016/05/19
CRDT- 2016/06/15 06:00
PHST- 2016/01/07 00:00 [received]
PHST- 2016/04/07 00:00 [revised]
PHST- 2016/04/18 00:00 [accepted]
PHST- 2016/06/15 06:00 [entrez]
PHST- 2016/06/15 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2016/05/19 00:00 [pmc-release]
AID - 10.1155/2016/9063842 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2016;2016:9063842. doi: 10.1155/2016/9063842. Epub 2016 May 
      19.