PMID- 2730077
OWN - NLM
STAT- MEDLINE
DCOM- 19890623
LR  - 20151119
IS  - 0385-0684 (Print)
IS  - 0385-0684 (Linking)
VI  - 16
IP  - 4 Pt 2-3
DP  - 1989 Apr
TI  - [Cell kinetic analysis with flow cytometry as a disdifferentiation mechanism in 
      human neuroblastoma cells in vitro].
PG  - 1842-50
AB  - The established cell line NB6 (human neuroblastoma) was perturbed in vitro, in 
      order to evaluate the disdifferentiation mechanism of malignant neoplastic cells 
      during cultivation. The methods for perturbation were as follows: 1) 50%-cell 
      removal from dishes; 2) 0.1 mg/ml of vincristine (VCR) contact; and 3) 10Gy of 
      beta-radiation. Specimens were harvested every 24 hours until 72 hours, 
      simultaneously stained for DNA and RNA with acridine orange (AO), and cell 
      kinetically analyzed for DNA/RNA/cell-distribution with the aid of flow cytometry 
      (FCM). Thus disdifferentiated cells and/or newly cell cycle traversing cells, 
      were evaluated. The new subpopulations were considered to be recruited from G1A 
      and/or Gq (Darzynkieowicz et al.) into the cell cycle after perturbations. Cells 
      of the new subpopulations had smaller volume compared with the control cells. 
      Furthermore, in the current study, newly cell cycle traversing cells of NB6 
      appeared to have a disturbed cell cycle traverse in G2B phase from the 
      clonological changing patterns of FCM cytograms. These new subpopulations were 
      revealed to be able to passage up to five or six times. Though it was impossible 
      to make them appear, these new cycling subpopulations might be capable of 
      remaining as permanent clones in vitro.
FAU - Yamamoto, H
AU  - Yamamoto H
AD  - Dept. of Neurosurgery, Tochigi Cancer Center.
FAU - Ichikawa, A
AU  - Ichikawa A
FAU - Shimamura, K
AU  - Shimamura K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Gan To Kagaku Ryoho
JT  - Gan to kagaku ryoho. Cancer & chemotherapy
JID - 7810034
RN  - 5J49Q6B70F (Vincristine)
SB  - IM
MH  - Beta Particles
MH  - *Cell Cycle/drug effects/radiation effects
MH  - Cell Differentiation
MH  - Cell Line
MH  - *Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - Neuroblastoma/*physiopathology
MH  - Vincristine/pharmacology
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
PST - ppublish
SO  - Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1842-50.