PMID- 27305770
OWN - NLM
STAT- MEDLINE
DCOM- 20160629
LR  - 20190923
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 12
IP  - 2
DP  - 2016 Feb
TI  - Micropatterned Culture and Differentiation of Human Bone Marrow Mesenchymal Stem 
      Cells Using a Polydimethylsiloxane Microstencil.
PG  - 366-70
AB  - A method for fabrication of polydimethylsiloxane (PDMS) microstencils was 
      developed and its application to localized culture of human bone marrow 
      mesenchymal stem cells (hMSCs) was tested. Unlike conventional culture methods, 
      which culture cells on an entire surface, microscale cell culture provides 
      precise control of the size and shape of stem cell patterns, and minimizes 
      consumption of cells and culture media. A PDMS microstencil was fabricated by 
      PDMS casting using an SU-8 mold prepared by photolithography. A pattern of 500-microm 
      dots was tested. For the test, a PDMS microstencil was placed on a glass disk and 
      cells were seeded on the stencil at a density of 5 x 10(4) cells/cm(2). The hMSCs 
      were cultured for 2 days at 37  degrees C in a humidified 5% CO2 atmosphere. The PDMS 
      microstencil was removed after 2 days and the hMSC patterns were inspected under 
      a microscope. The results confirmed that stem cells can be cultured using a PDMS 
      microstencil. The micropatterned hMSCs retained their ability to differentiate 
      into osteogenic and adipogenic cells. Thus, using a PDMS microstencil, stem cells 
      can be cultured and differentiated in micropatterns in a precisely controlled 
      manner, in any shape and size, for research and bioengineering applications.
FAU - Choi, Jin Ho
AU  - Choi JH
FAU - Bae, Jae-Sung
AU  - Bae JS
FAU - Lee, Hyun
AU  - Lee H
FAU - Jin, Hee Kyung
AU  - Jin HK
FAU - Kim, Gyu Man
AU  - Kim GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Dimethylpolysiloxanes)
SB  - IM
MH  - Bone Marrow Cells/*cytology
MH  - Cell Culture Techniques/*methods
MH  - *Cell Differentiation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microscopy, Electron, Scanning
MH  - Microtechnology/*instrumentation/*methods
EDAT- 2016/06/17 06:00
MHDA- 2016/06/30 06:00
CRDT- 2016/06/17 06:00
PHST- 2016/06/17 06:00 [entrez]
PHST- 2016/06/17 06:00 [pubmed]
PHST- 2016/06/30 06:00 [medline]
AID - 10.1166/jbn.2016.2186 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2016 Feb;12(2):366-70. doi: 10.1166/jbn.2016.2186.