PMID- 27306996
OWN - NLM
STAT- MEDLINE
DCOM- 20170223
LR  - 20181113
IS  - 1433-4909 (Electronic)
IS  - 1431-0651 (Linking)
VI  - 20
IP  - 5
DP  - 2016 Sep
TI  - Comparative analysis of prokaryotic diversity in solar salterns in eastern 
      Anatolia (Turkey).
PG  - 589-601
LID - 10.1007/s00792-016-0845-7 [doi]
AB  - The prokaryotic communities of four salterns (Bingol, Fadlum, Kemah, and 
      Tuzlagozu) in Turkey were examined and compared using the cultivation and 
      cultivation-independent methods [fluorescence in situ hybridization (FISH) and 
      454 pyrosequencing]. FISH analysis with universal probes revealed that feeding 
      waters carried 1.6 x 10(2)-1.7 x 10(3) cells mL(-1), while crystallization ponds 
      carried 3.8 x 10(6)-2.0 x 10(7) cells mL(-1) that were mostly haloarchaea, 
      including square cells (except for Kemah). High-throughput 16S rRNA-based gene 
      sequencing showed that the most frequent archaeal OTUs in Bingol, Fadlum, 
      Tuzlagozu, and Kemah samples were affiliated with Haloquadratum (76.8 %), 
      Haloarcula (27.8 %), Halorubrum (49.6 %), and Halonotius (59.8 %), respectively. 
      Bacteroidetes was the dominant bacterial phylum in Bingol and Fadlum, 
      representing 71.5 and 79.5 % of the bacterial OTUs (respectively), while the most 
      abundant bacterial phylum found in the Kemah saltern was Proteobacteria (79.6 %). 
      The majority of the bacterial OTUs recovered from Tuzlagozu belonged to the 
      Cyanobacteria (35.7 %), Bacteroidetes (35.0 %), and Proteobacteria (25.5 %) 
      phyla. Cultivation studies revealed that the archaeal isolates were closely 
      related to the genera Halobacterium, Haloarcula, and Halorubrum. Bacterial 
      isolates were confined to two phyla, Proteobacteria (Alphaproteobacteria and 
      Gammaproteobacteria classes) and Bacteroidetes. Comparative analysis showed that 
      members of the Euryarchaeota, Bacteroidetes, Proteobacteria, and Cyanobacteria 
      phyla were major inhabitants of the solar salterns.
FAU - Cinar, Seval
AU  - Cinar S
AD  - Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, 
      Turkey. sevalcinar@anadolu.edu.tr.
FAU - Mutlu, Mehmet Burcin
AU  - Mutlu MB
AD  - Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20160615
PL  - Germany
TA  - Extremophiles
JT  - Extremophiles : life under extreme conditions
JID - 9706854
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteroidetes/classification/genetics/isolation & purification
MH  - Euryarchaeota/classification/genetics/isolation & purification
MH  - *Microbiota
MH  - Phylogeny
MH  - Proteobacteria/classification/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Saline Waters
MH  - Salinity
MH  - Turkey
MH  - *Water Microbiology
OTO - NOTNLM
OT  - FISH
OT  - Halophilic Archaea/Bacteria
OT  - Pyrosequencing
OT  - Solar saltern
EDAT- 2016/06/17 06:00
MHDA- 2017/02/24 06:00
CRDT- 2016/06/17 06:00
PHST- 2015/12/23 00:00 [received]
PHST- 2016/05/31 00:00 [accepted]
PHST- 2016/06/17 06:00 [entrez]
PHST- 2016/06/17 06:00 [pubmed]
PHST- 2017/02/24 06:00 [medline]
AID - 10.1007/s00792-016-0845-7 [pii]
AID - 10.1007/s00792-016-0845-7 [doi]
PST - ppublish
SO  - Extremophiles. 2016 Sep;20(5):589-601. doi: 10.1007/s00792-016-0845-7. Epub 2016 
      Jun 15.