PMID- 27307043
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20210205
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 291
IP  - 32
DP  - 2016 Aug 5
TI  - Structural and Functional Attributes of the Interleukin-36 Receptor.
PG  - 16597-609
LID - 10.1074/jbc.M116.723064 [doi]
AB  - Signal transduction by the IL-36 receptor (IL-36R) is linked to several human 
      diseases. However, the structure and function of the IL-36R is not well 
      understood. A molecular model of the IL-36R complex was generated and a 
      cell-based reporter assay was established to assess the signal transduction of 
      recombinant subunits of the IL-36R. Mutational analyses and functional assays 
      have identified residues of the receptor subunit IL-1Rrp2 needed for cytokine 
      recognition, stable protein expression, disulfide bond formation and 
      glycosylation that are critical for signal transduction. We also observed that, 
      overexpression of ectodomain (ECD) of Il-1Rrp2 or IL-1RAcP exhibited 
      dominant-negative effect on IL-36R signaling. The presence of IL-36 cytokine 
      significantly increased the interaction of IL-1Rrp2 ECD with the co-receptor 
      IL-1RAcP. Finally, we found that single nucleotide polymorphism A471T in the 
      Toll-interleukin 1 receptor domain (TIR) of the IL-1Rrp2 that is present in  approximately 2% 
      of the human population, down-regulated IL-36R signaling by a decrease of 
      interaction with IL-1RAcP.
CI  - (c) 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Yi, Guanghui
AU  - Yi G
AD  - From the Departments of Molecular and Cellular Biochemistry and.
FAU - Ybe, Joel A
AU  - Ybe JA
AD  - Environmental Health, School of Public Health, Indiana University, Bloomington, 
      Indiana 47405 and.
FAU - Saha, Siddhartha S
AU  - Saha SS
AD  - From the Departments of Molecular and Cellular Biochemistry and.
FAU - Caviness, Gary
AU  - Caviness G
AD  - Boehringer Ingelheim Pharmaceuticals, Ridgefield, Connecticut 06877.
FAU - Raymond, Ernest
AU  - Raymond E
AD  - Boehringer Ingelheim Pharmaceuticals, Ridgefield, Connecticut 06877.
FAU - Ganesan, Rajkumar
AU  - Ganesan R
AD  - Boehringer Ingelheim Pharmaceuticals, Ridgefield, Connecticut 06877.
FAU - Mbow, M Lamine
AU  - Mbow ML
AD  - Boehringer Ingelheim Pharmaceuticals, Ridgefield, Connecticut 06877.
FAU - Kao, C Cheng
AU  - Kao CC
AD  - From the Departments of Molecular and Cellular Biochemistry and ckao@indiana.edu.
LA  - eng
SI  - PDB/4DEP
PT  - Journal Article
DEP - 20160615
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (IL18R1 protein, human)
RN  - 0 (IL1RAP protein, human)
RN  - 0 (Interleukin-1 Receptor Accessory Protein)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
SB  - IM
MH  - HEK293 Cells
MH  - Humans
MH  - *Interleukin-1 Receptor Accessory Protein/chemistry/genetics/metabolism
MH  - *Interleukin-18 Receptor alpha Subunit/chemistry/genetics/metabolism
MH  - *Polymorphism, Genetic
MH  - Protein Domains
MH  - Signal Transduction
MH  - Structure-Activity Relationship
PMC - PMC4974375
OTO - NOTNLM
OT  - cytokine
OT  - immunology
OT  - membrane protein
OT  - psoriasis
OT  - receptor structure-function
OT  - signal transduction
EDAT- 2016/06/17 06:00
MHDA- 2017/05/06 06:00
PMCR- 2017/08/05
CRDT- 2016/06/17 06:00
PHST- 2016/02/22 00:00 [received]
PHST- 2016/06/17 06:00 [entrez]
PHST- 2016/06/17 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
PHST- 2017/08/05 00:00 [pmc-release]
AID - S0021-9258(20)35383-7 [pii]
AID - M116.723064 [pii]
AID - 10.1074/jbc.M116.723064 [doi]
PST - ppublish
SO  - J Biol Chem. 2016 Aug 5;291(32):16597-609. doi: 10.1074/jbc.M116.723064. Epub 
      2016 Jun 15.