PMID- 27307570
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20181113
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 90
IP  - 17
DP  - 2016 Sep 1
TI  - Antiviral Activity of Myticin C Peptide from Mussel: an Ancient Defense against 
      Herpesviruses.
PG  - 7692-702
LID - 10.1128/JVI.00591-16 [doi]
AB  - Little is known about the antiviral response in mollusks. As in other 
      invertebrates, the interferon signaling pathways have not been identified, and in 
      fact, there is a debate about whether invertebrates possess antiviral immunity 
      similar to that of vertebrates. In marine bivalves, due to their filtering 
      activity, interaction with putative pathogens, including viruses, is very high, 
      suggesting that they should have mechanisms to address these infections. In this 
      study, we confirmed that constitutively expressed molecules in naive mussels 
      confer resistance in oysters to ostreid herpesvirus 1 (OsHV-1) when oyster 
      hemocytes are incubated with mussel hemolymph. Using a proteomic approach, 
      myticin C peptides were identified in both mussel hemolymph and hemocytes. 
      Myticins, antimicrobial peptides that have been previously characterized, were 
      constitutively expressed in a fraction of mussel hemocytes and showed antiviral 
      activity against OsHV-1, suggesting that these molecules could be responsible for 
      the antiviral activity of mussel hemolymph. For the first time, a molecule from a 
      bivalve has shown antiviral activity against a virus affecting mollusks. 
      Moreover, myticin C peptides showed antiviral activity against human herpes 
      simplex viruses 1 (HSV-1) and 2 (HSV-2). In summary, our work sheds light on the 
      invertebrate antiviral immune response with the identification of a molecule with 
      potential biotechnological applications. IMPORTANCE: Several bioactive molecules 
      that have potential pharmaceutical or industrial applications have been 
      identified and isolated from marine invertebrates. Myticin C, an antimicrobial 
      peptide from the Mediterranean mussel (Mytilus galloprovincialis) that was 
      identified by proteomic techniques in both mussel hemolymph and hemocytes, showed 
      potential as an antiviral agent against ostreid herpesvirus 1 (OsHV-1), which 
      represents a major threat to the oyster-farming sector. Both hemolymph from 
      mussels and a myticin C peptide inhibited OsHV-1 replication in oyster hemocytes. 
      Additionally, a modified peptide derived from myticin C or the nanoencapsulated 
      normal peptide also showed antiviral activity against the human herpesviruses 
      HSV-1 and HSV-2. Therefore, myticin C is an example of the biotechnological and 
      therapeutic potential of mollusks.
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Novoa, Beatriz
AU  - Novoa B
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain beatriznovoa@iim.csic.es.
FAU - Romero, Alejandro
AU  - Romero A
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain.
FAU - Alvarez, Angel L
AU  - Alvarez AL
AUID- ORCID: 0000-0001-9388-8595
AD  - Instituto Universitario de Biotecnologia de Asturias (IUBA), Departamento de 
      Bioquimica y Biologia Molecular, Universidad de Oviedo, Oviedo, Asturias, Spain.
FAU - Moreira, Rebeca
AU  - Moreira R
AUID- ORCID: 0000-0001-7797-7221
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain.
FAU - Pereiro, Patricia
AU  - Pereiro P
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain.
FAU - Costa, Maria M
AU  - Costa MM
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain.
FAU - Dios, Sonia
AU  - Dios S
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain.
FAU - Estepa, Amparo
AU  - Estepa A
AD  - Instituto de Biologia Molecular y Celular (IBMC), Universidad Miguel Hernandez, 
      Elche, Spain.
FAU - Parra, Francisco
AU  - Parra F
AD  - Instituto Universitario de Biotecnologia de Asturias (IUBA), Departamento de 
      Bioquimica y Biologia Molecular, Universidad de Oviedo, Oviedo, Asturias, Spain.
FAU - Figueras, Antonio
AU  - Figueras A
AD  - Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones 
      Cientificas (CSIC), Vigo, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160812
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Antiviral Agents)
RN  - 0 (Biological Products)
RN  - 0 (Blood Proteins)
RN  - 0 (myticin)
SB  - IM
MH  - Animals
MH  - Antimicrobial Cationic Peptides/isolation & purification/*metabolism
MH  - Antiviral Agents/isolation & purification/*metabolism
MH  - Biological Products/isolation & purification/*metabolism
MH  - Bivalvia/*chemistry
MH  - Blood Proteins/isolation & purification/*metabolism
MH  - Herpesviridae/*drug effects
MH  - Humans
MH  - Virus Replication/drug effects
PMC - PMC4988142
EDAT- 2016/06/17 06:00
MHDA- 2017/05/10 06:00
PMCR- 2017/02/12
CRDT- 2016/06/17 06:00
PHST- 2016/03/30 00:00 [received]
PHST- 2016/06/08 00:00 [accepted]
PHST- 2016/06/17 06:00 [entrez]
PHST- 2016/06/17 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2017/02/12 00:00 [pmc-release]
AID - JVI.00591-16 [pii]
AID - 00591-16 [pii]
AID - 10.1128/JVI.00591-16 [doi]
PST - epublish
SO  - J Virol. 2016 Aug 12;90(17):7692-702. doi: 10.1128/JVI.00591-16. Print 2016 Sep 
      1.