PMID- 27309037
OWN - NLM
STAT- MEDLINE
DCOM- 20170224
LR  - 20170224
IS  - 1827-1669 (Electronic)
IS  - 0026-4806 (Linking)
VI  - 107
IP  - 6
DP  - 2016 Dec
TI  - Epigallocatechin gallate suppresses Chlamydia pneumoniae mediated IgE responses 
      in peripheral blood mononuclear cells: a pilot study.
PG  - 363-369
AB  - BACKGROUND: Chlamydia pneumoniae (C. pneumoniae) causes respiratory infection in 
      children and adults and is associated with asthma and induction of immunoglobulin 
      E (IgE) responses. Previous studies in our laboratory reported that green tea 
      extract (GTE) and its catechin, epigallocatechin gallate (EGCG) have 
      immunoregulatory effects on IgE responses. Whereas tea polyphenols have in vitro 
      inhibitory effects on the proliferation of C. pneumoniae, the in vitro effects of 
      EGCG on C. pneumoniae- mediated IgE responses haven't been studied. We sought to 
      clarify the in vitro effect of EGCG on C. pneumoniae mediated IgE responses by 
      peripheral blood mononuclear cells (PBMC) in asthma. METHODS: PBMC from subjects 
      with asthma and non-asthmatic controls were incubated with C. pneumoniae and 
      cultured for 10 days +/-EGCG (0.5, 5.0, 50 ng/mL). IgE levels in supernatants were 
      determined (ELISA). RESULTS: Elevated IgE levels were detected in supernatants of 
      PBMC from an asthma patient (2.6 ng/mL), whereas IgE levels of PBMC from 
      non-asthmatics were low (<2.0 ng/mL) at baseline. When EGCG (0.5-50 ng/mL) was 
      added to PBMC from the asthma patient, IgE production was suppressed in a 
      dose-dependent manner (10-30%), compared with no EGCG. When PBMC from the asthma 
      patient were incubated with C. pneumoniae, IgE production was suppressed (70%); 
      when PBMC from non-asthmatics were incubated with C. pneumoniae, IgE levels 
      remained undetectable (<2.0 ng/mL). When EGCG (0.5-50 ng/mL) was added to PBMC 
      from the asthma patient, C. pneumoniae-induced IgE production was suppressed 
      moderately (35-48%). CONCLUSIONS: EGCG suppressed C. pneumoniae- mediated IgE 
      responses in PBMC from a patient with asthma.
FAU - Smith-Norowitz, Tamar A
AU  - Smith-Norowitz TA
AD  - Division of Infectious Diseases, Department of Pediatrics, State University of 
      New York Downstate Medical Center, Brooklyn, New York, NY, USA - 
      tamar.smith-norowitz@downstate.edu.
FAU - Chotikanatis, Kobkul
AU  - Chotikanatis K
FAU - Tam, Elizabeth
AU  - Tam E
FAU - Norowitz, Yitzchok M
AU  - Norowitz YM
FAU - Joks, Rauno
AU  - Joks R
FAU - Durkin, Helen G
AU  - Durkin HG
FAU - Hammerschlag, Margaret R
AU  - Hammerschlag MR
FAU - Kohlhoff, Stephan
AU  - Kohlhoff S
LA  - eng
PT  - Journal Article
DEP - 20160616
PL  - Italy
TA  - Minerva Med
JT  - Minerva medica
JID - 0400732
RN  - 0 (Antioxidants)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
SB  - IM
MH  - Adult
MH  - Antioxidants/*pharmacology
MH  - Asthma/blood
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Cells, Cultured
MH  - Chlamydophila pneumoniae/*immunology
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*drug effects/*physiology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Male
MH  - Pilot Projects
MH  - Young Adult
EDAT- 2016/06/17 06:00
MHDA- 2017/02/25 06:00
CRDT- 2016/06/17 06:00
PHST- 2016/06/17 06:00 [pubmed]
PHST- 2017/02/25 06:00 [medline]
PHST- 2016/06/17 06:00 [entrez]
AID - R10Y9999N00A16061602 [pii]
PST - ppublish
SO  - Minerva Med. 2016 Dec;107(6):363-369. Epub 2016 Jun 16.