PMID- 27313153
OWN - NLM
STAT- MEDLINE
DCOM- 20170516
LR  - 20170516
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 27
IP  - 3-4
DP  - 2016 May
TI  - Simultaneous Quantification of Seven Bioactive Flavonoids in Citri Reticulatae 
      Pericarpium by Ultra-Fast Liquid Chromatography Coupled with Tandem Mass 
      Spectrometry.
PG  - 168-73
LID - 10.1002/pca.2612 [doi]
AB  - INTRODUCTION: Citri Reticulatae Pericarpium (CRP) is a commonly-used traditional 
      Chinese medicine with flavonoids as the major bioactive components. Nevertheless, 
      the contents of the flavonoids in CRP of different sources may significantly vary 
      affecting their therapeutic effects. Thus, the setting up of a reliable and 
      comprehensive quality assessment method for flavonoids in CRP is necessary. 
      OBJECTIVE: To set up a rapid and sensitive ultra-fast liquid chromatography 
      coupled with tandem mass spectrometry (UFLC-MS/MS) method for simultaneous 
      quantification of seven bioactive flavonoids in CRP. METHODS: A UFLC-MS/MS method 
      coupled to ultrasound-assisted extraction was developed for simultaneous 
      separation and quantification of seven flavonoids including hesperidin, 
      neohesperidin, naringin, narirutin, tangeretin, nobiletin and sinensetin in 16 
      batches of CRP samples from different sources in China. RESULTS: The established 
      method showed good linearity for all analytes with correlation coefficient (R) 
      over 0.9980, together with satisfactory accuracy, precision and reproducibility. 
      Furthermore, the recoveries at the three spiked levels were higher than 89.71% 
      with relative standard deviations (RSDs) lower than 5.19%. The results indicated 
      that the contents of seven bioactive flavonoids in CRP varied significantly among 
      different sources. Among the samples under study, hesperidin showed the highest 
      contents in 16 samples ranged from 27.50 to 86.30 mg/g, the contents of 
      hesperidin in CRP-15 and CRP-9 were 27.50 and 86.30 mg/g, respectively, while, 
      the amount of narirutin was too low to be measured in some samples. CONCLUSION: 
      This study revealed that the developed UFLC-MS/MS method was simple, sensitive 
      and reliable for simultaneous quantification of multi-components in CRP with 
      potential perspective for quality control of complex matrices. Copyright (c) 2016 
      John Wiley & Sons, Ltd.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Zhao, Lian-Hua
AU  - Zhao LH
AD  - Jilin Agricultural University, Changchun, 130118, China.
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Zhao, Hong-Zheng
AU  - Zhao HZ
AD  - Jilin Agricultural University, Changchun, 130118, China.
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Zhao, Xue
AU  - Zhao X
AD  - Jilin Agricultural University, Changchun, 130118, China.
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing, 100193, China.
AD  - Jilin Agriculture Science and Technology College, Jilin, 132101, China.
FAU - Kong, Wei-Jun
AU  - Kong WJ
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Hu, Yi-Chen
AU  - Hu YC
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Yang, Shi-Hai
AU  - Yang SH
AD  - Jilin Agricultural University, Changchun, 130118, China.
FAU - Yang, Mei-Hua
AU  - Yang MH
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing, 100193, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
SB  - IM
MH  - China
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Citrus/*chemistry
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Flavonoids/*chemistry
MH  - Medicine, Chinese Traditional
MH  - Molecular Structure
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Citri Reticulatae Pericarpium
OT  - UFLC-MS/MS
OT  - bioactive flavonoids
OT  - simultaneous quantification
OT  - ultrasound-assisted extraction
EDAT- 2016/06/18 06:00
MHDA- 2017/05/17 06:00
CRDT- 2016/06/18 06:00
PHST- 2015/05/18 00:00 [received]
PHST- 2015/12/30 00:00 [revised]
PHST- 2016/01/03 00:00 [accepted]
PHST- 2016/06/18 06:00 [entrez]
PHST- 2016/06/18 06:00 [pubmed]
PHST- 2017/05/17 06:00 [medline]
AID - 10.1002/pca.2612 [doi]
PST - ppublish
SO  - Phytochem Anal. 2016 May;27(3-4):168-73. doi: 10.1002/pca.2612.