PMID- 27314631
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20221207
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 159
DP  - 2016 Sep
TI  - Effects of organic matter in livestock manure digester liquid on microbial 
      community structure and in situ activity of anammox granules.
PG  - 300-307
LID - S0045-6535(16)30769-X [pii]
LID - 10.1016/j.chemosphere.2016.06.018 [doi]
AB  - Anaerobic ammonium oxidation (anammox) is a promising process for NH4(+)-rich 
      wastewaters such as anaerobic digester liquids. In the present study, we 
      investigated various properties of an up-flow column reactor containing anammox 
      granules and fed with a real digester liquid at four different concentrations 
      (Phases 1 to 4). The efficiencies of NH4(+) and NO2(-) removal decreased by up to 
      32% and 42%, respectively, in the digester-liquid-fed reactor (reactor-DL). When 
      the performance of reactor-DL deteriorated, the community structure, spatial 
      distribution, and in situ anammox activity in the two reactors were further 
      investigated using 16S rRNA gene-based phylogenetic analysis, fluorescence in 
      situ hybridization (FISH), and microelectrode measurements. The phylogenetic 
      analysis and FISH results showed that non-anammox bacteria were predominant in 
      the granule outer layers in reactor-DL, whereas anammox bacteria still dominated 
      the granule interiors. Microelectrode measurements showed clear evidence of 
      NH4(+) oxidation activity in the interiors of granules from reactor-DL. Batch 
      experiments using anammox granules at different acetate concentrations indicated 
      that concentrations up to 50 mM had no effects on the anammox activity, whereas 
      inorganic carbon uptake decreased in the presence of acetate. The present study 
      clearly shows that the anammox activity and anammox bacterial density in the 
      granules were maintained after feeding the digester liquid to the reactor for 140 
      days.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Kindaichi, Tomonori
AU  - Kindaichi T
AD  - Department of Civil and Environmental Engineering, Hiroshima University, 1-4-1 
      Kagamiyama, Higashihiroshima 739-8527, Japan. Electronic address: 
      tomokin@hiroshima-u.ac.jp.
FAU - Awata, Takanori
AU  - Awata T
AD  - Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
FAU - Mugimoto, Yuichiro
AU  - Mugimoto Y
AD  - Tokyo Engineering Consultants Co., Ltd., 3-7-1 Kasumigaseki, Chiyoda-ku, Tokyo 
      100-0013, Japan.
FAU - Rathnayake, Rathnayake M L D
AU  - Rathnayake RMLD
AD  - Division of Environmental Engineering, Faculty of Engineering, Hokkaido 
      University, Kita-13, Nishi-8, Kita-ku, Sapporo 060-8628, Japan.
FAU - Kasahara, Shinsuke
AU  - Kasahara S
AD  - Department of Environmental Engineering, Osaka Institute of Technology, 5-16-1 
      Omiya, Asahi-ku, Osaka 535-8585, Japan.
FAU - Satoh, Hisashi
AU  - Satoh H
AD  - Division of Environmental Engineering, Faculty of Engineering, Hokkaido 
      University, Kita-13, Nishi-8, Kita-ku, Sapporo 060-8628, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160614
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Manure)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
RN  - 0 (Water Pollutants, Chemical)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Anaerobiosis
MH  - Animals
MH  - Bacteria/genetics/*metabolism
MH  - *Biodegradation, Environmental
MH  - Bioreactors/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Livestock
MH  - Manure
MH  - Microbiota/*genetics
MH  - Nitrogen/analysis
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - Quaternary Ammonium Compounds/analysis/*chemistry
MH  - RNA, Ribosomal, 16S/genetics
MH  - Wastewater/analysis/*chemistry
MH  - Water Pollutants, Chemical/*chemistry
OTO - NOTNLM
OT  - Anaerobic ammonium oxidation (anammox)
OT  - Digester liquid
OT  - Fluorescence in situ hybridization (FISH)
OT  - Microelectrodes
OT  - Phylogenetic analysis
EDAT- 2016/06/18 06:00
MHDA- 2017/03/14 06:00
CRDT- 2016/06/18 06:00
PHST- 2016/02/24 00:00 [received]
PHST- 2016/05/23 00:00 [revised]
PHST- 2016/06/05 00:00 [accepted]
PHST- 2016/06/18 06:00 [entrez]
PHST- 2016/06/18 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
AID - S0045-6535(16)30769-X [pii]
AID - 10.1016/j.chemosphere.2016.06.018 [doi]
PST - ppublish
SO  - Chemosphere. 2016 Sep;159:300-307. doi: 10.1016/j.chemosphere.2016.06.018. Epub 
      2016 Jun 14.