PMID- 27314642
OWN - NLM
STAT- MEDLINE
DCOM- 20180810
LR  - 20181113
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 88
IP  - 13
DP  - 2016 Jul 5
TI  - Profiling Reactive Metabolites via Chemical Trapping and Targeted Mass 
      Spectrometry.
PG  - 6658-61
LID - 10.1021/acs.analchem.6b02009 [doi]
AB  - Metabolomic profiling studies aim to provide a comprehensive, quantitative, and 
      dynamic portrait of the endogenous metabolites in a biological system. While 
      contemporary technologies permit routine profiling of many metabolites, 
      intrinsically labile metabolites are often improperly measured or omitted from 
      studies due to unwanted chemical transformations that occur during sample 
      preparation or mass spectrometric analysis. The primary glycolytic metabolite 
      1,3-bisphosphoglyceric acid (1,3-BPG) typifies this class of metabolites, and, 
      despite its central position in metabolism, has largely eluded analysis in 
      profiling studies. Here we take advantage of the reactive acylphosphate group in 
      1,3-BPG to chemically trap the metabolite with hydroxylamine during metabolite 
      isolation, enabling quantitative analysis by targeted LC-MS/MS. This approach is 
      compatible with complex cellular metabolome, permits specific detection of the 
      reactive (1,3-) instead of nonreactive (2,3-) BPG isomer, and has enabled direct 
      analysis of dynamic 1,3-BPG levels resulting from perturbations to glucose 
      processing. These studies confirmed that standard metabolomic methods 
      misrepresent cellular 1,3-BPG levels in response to altered glucose metabolism 
      and underscore the potential for chemical trapping to be used for other classes 
      of reactive metabolites.
FAU - Chang, Jae Won
AU  - Chang JW
AD  - Department of Chemistry and double daggerInstitute for Genomics and Systems Biology, 
      University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United 
      States.
FAU - Lee, Gihoon
AU  - Lee G
AD  - Department of Chemistry and double daggerInstitute for Genomics and Systems Biology, 
      University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United 
      States.
FAU - Coukos, John S
AU  - Coukos JS
AD  - Department of Chemistry and double daggerInstitute for Genomics and Systems Biology, 
      University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United 
      States.
FAU - Moellering, Raymond E
AU  - Moellering RE
AD  - Department of Chemistry and double daggerInstitute for Genomics and Systems Biology, 
      University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United 
      States.
LA  - eng
GR  - R00 CA175399/CA/NCI NIH HHS/United States
GR  - T32 GM007281/GM/NIGMS NIH HHS/United States
PT  - Letter
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160623
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Diphosphoglyceric Acids)
RN  - 1981-49-3 (glycerate 1,3-biphosphate)
RN  - 2FP81O2L9Z (Hydroxylamine)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromatography, High Pressure Liquid
MH  - Diphosphoglyceric Acids/*chemistry/metabolism
MH  - Glucose/chemistry/*metabolism
MH  - Humans
MH  - Hydroxylamine/*chemistry
MH  - Isomerism
MH  - *Metabolome
MH  - *Tandem Mass Spectrometry
PMC - PMC4998964
MID - NIHMS796719
EDAT- 2016/06/18 06:00
MHDA- 2018/08/11 06:00
PMCR- 2017/07/05
CRDT- 2016/06/18 06:00
PHST- 2016/06/18 06:00 [entrez]
PHST- 2016/06/18 06:00 [pubmed]
PHST- 2018/08/11 06:00 [medline]
PHST- 2017/07/05 00:00 [pmc-release]
AID - 10.1021/acs.analchem.6b02009 [doi]
PST - ppublish
SO  - Anal Chem. 2016 Jul 5;88(13):6658-61. doi: 10.1021/acs.analchem.6b02009. Epub 
      2016 Jun 23.