PMID- 27316386 OWN - NLM STAT- MEDLINE DCOM- 20180405 LR - 20181202 IS - 2452-3186 (Electronic) IS - 2452-3186 (Linking) VI - 64 IP - 2 DP - 2016 Apr-Jun TI - No significant effects of Poly(I:C) on human umbilical cord-derived mesenchymal stem cells in the treatment of B6.MRL-Fas(lpr) mice. PG - 55-60 LID - S2452-3186(16)00034-9 [pii] LID - 10.1016/j.retram.2016.03.002 [doi] AB - OBJECTIVE: Our study aimed to compare the curative effect and immunoregulation between MSCs activated by Poly(I:C) for 24hours and unactivated MSCs on lupus mice. MATERIALS AND METHODS: MSCs were pretreated by Poly(I:C) at 50mug/mL for 24h. B6.MRL-Fas(lpr) mice were divided into UC-MSC treated group, FLS treated group, Poly(I:C) preconditioned MSC treated group (P-MSC) and untreated group randomly. All treated mice were infused with 1x10(6) MSCs or FLSs at the 24th week and were sacrificed 4 weeks later. The spleen weight, serum immunoglobulin G (IgG) levels, serum anti-double stranded DNA (anti-dsDNA) antibody levels, immune cell subsets, renal lesions and IgG deposition in the kidney were evaluated. The effects of two kinds of MSCs on the proliferation and apoptosis of CD4+ T cells were detected by flow cytometry. The TLR3 expression at protein level in MSCs was assessed with and without Poly(I:C) treatment. The expression of immunoregulatory factors were detected by qRT-PCR in different dose and duration of Poly(I:C). RESULT: Poly(I:C) preconditioned MSCs had similar therapeutic effects in lupus mice compared with untreated MSCs in vivo. Furthermore, Poly(I:C) treated MSCs and untreated MSCs had comparable inhibitory effects on proliferation of T cells, and Poly(I:C) could enhance the expression of TLR3 at protein and mRNA level. Poly(I:C) could partly alter the mRNA levels of immunoregulatory factors, such as hepatocyte growth factor, transforming growth factor beta1, vascular endothelial growth factor, but did not have significant changes in cyclooxygenase 2, interleukin 6, tumor necrosis factor alpha, indoleamine 2,3-dioxygenase, interferon gamma and chemokine (C-C motif) ligand 2. CONCLUSION: Our study did not find that Poly(I:C) treatment could enhance the therapeutic effect of MSCs in lupus mice in vivo. CI - Copyright (c) 2016. Published by Elsevier Masson SAS. FAU - Huang, S AU - Huang S AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Wang, D AU - Wang D AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Gu, F AU - Gu F AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Zhang, Z AU - Zhang Z AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Deng, W AU - Deng W AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Chen, W AU - Chen W AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Tang, X AU - Tang X AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Yao, G AU - Yao G AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Feng, X AU - Feng X AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. FAU - Sun, L AU - Sun L AD - The Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Rheumatology and Immunology, 321, Zhongshan Road, Nanjing, Jiangsu 210008, China. Electronic address: lingyunsun@nju.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160603 PL - France TA - Curr Res Transl Med JT - Current research in translational medicine JID - 101681234 RN - 0 (Antibodies, Antinuclear) RN - 0 (Immunoglobulin G) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (RNA, Messenger) RN - 0 (TLR3 protein, mouse) RN - 0 (Toll-Like Receptor 3) RN - O84C90HH2L (Poly I-C) SB - IM MH - Animals MH - Antibodies, Antinuclear/analysis MH - Cells, Cultured MH - Drug Evaluation, Preclinical MH - Female MH - Heterografts MH - Humans MH - Immunoglobulin G/analysis MH - Intercellular Signaling Peptides and Proteins/biosynthesis/genetics MH - Kidney/immunology/pathology MH - Lupus Erythematosus, Systemic/pathology/*therapy MH - *Mesenchymal Stem Cell Transplantation MH - Mesenchymal Stem Cells/*drug effects MH - Mice MH - Mice, Inbred MRL lpr MH - Poly I-C/*pharmacology MH - RNA, Messenger/biosynthesis MH - Random Allocation MH - Spleen/pathology MH - Toll-Like Receptor 3/biosynthesis/genetics MH - Umbilical Cord/*cytology OTO - NOTNLM OT - Lupus OT - Mesenchymal stem cells OT - Poly-inosinic acid:cytidylic acid OT - Toll-like receptor 3 EDAT- 2016/06/19 06:00 MHDA- 2018/04/06 06:00 CRDT- 2016/06/19 06:00 PHST- 2015/10/13 00:00 [received] PHST- 2016/03/11 00:00 [accepted] PHST- 2016/06/19 06:00 [entrez] PHST- 2016/06/19 06:00 [pubmed] PHST- 2018/04/06 06:00 [medline] AID - S2452-3186(16)00034-9 [pii] AID - 10.1016/j.retram.2016.03.002 [doi] PST - ppublish SO - Curr Res Transl Med. 2016 Apr-Jun;64(2):55-60. doi: 10.1016/j.retram.2016.03.002. Epub 2016 Jun 3.