PMID- 27316436
OWN - NLM
STAT- MEDLINE
DCOM- 20170105
LR  - 20220331
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 129
IP  - 10
DP  - 2016 Oct
TI  - Mapping quantitative trait loci conferring resistance to a widely virulent 
      isolate of Cochliobolus sativus in wild barley accession PI 466423.
PG  - 1831-42
LID - 10.1007/s00122-016-2742-y [doi]
AB  - This research characterized the genetics of resistance of wild barley accession 
      PI 466423 to a widely virulent pathotype of Cochliobolus sativus . Breeding lines 
      were identified that combine the Midwest Six-rowed Durable Resistance Haplotype 
      and resistance to the virulent isolate ND4008. Spot blotch, caused by 
      Cochliobolus sativus, is a historically important foliar disease of barley 
      (Hordeum vulgare L.) in the Upper Midwest region of the USA. However, for the 
      last 50 years this disease has been of little consequence due to the deployment 
      of resistant six-rowed malting cultivars. These durably resistant cultivars carry 
      the Midwest Six-rowed Durable Resistant Haplotype (MSDRH) comprised of three 
      Quantitative Trait Loci (QTL) on chromosomes 1H, 3H and 7H, originally 
      contributed by breeding line NDB112. Recent reports of C. sativus isolates (e.g. 
      ND4008) with virulence on NDB112 indicate that widely grown cultivars of the 
      region are vulnerable to spot blotch epidemics. Wild barley (H. vulgare ssp. 
      spontaneum), the progenitor of cultivated barley, is a rich source of novel 
      alleles, especially for disease resistance. Wild barley accession PI 466423 is 
      highly resistant to C. sativus isolate ND4008. To determine the genetic 
      architecture of resistance to isolate ND4008 in PI 466423, we phenotyped and 
      genotyped an advanced backcross population (N = 244) derived from the wild 
      accession and the recurrent parent 'Rasmusson', a Minnesota cultivar with the 
      MSDRH. Disease phenotyping was done on BC2F4 seedlings in the greenhouse using 
      isolate ND4008. The Rasmusson/PI 466423 population was genotyped with 7842 single 
      nucleotide polymorphic markers. QTL analysis using composite interval mapping 
      revealed four resistance loci on chromosomes 1H, 2H, 4H and 5H explaining 10.3, 
      7.4, 6.4 and 8.4 % of the variance, respectively. Resistance alleles on 
      chromosomes 1H, 4H and 5H were contributed by PI 466423, whereas the one on 
      chromosome 2H was contributed by Rasmusson. All four resistance QTL are likely 
      coincident with previously identified QTL. Agronomically advanced two- and 
      six-rowed lines combining the MSDRH and resistance alleles to isolate ND4008 have 
      been identified and are being utilized in breeding. These results reaffirm the 
      value of using wild relatives as a source of novel resistance alleles.
FAU - Haas, Matthew
AU  - Haas M
AD  - Department of Plant Pathology, University of Minnesota, Saint Paul, MN, 55108, 
      USA.
FAU - Menke, Jon
AU  - Menke J
AD  - Department of Plant Pathology, University of Minnesota, Saint Paul, MN, 55108, 
      USA.
FAU - Chao, Shiaoman
AU  - Chao S
AD  - Cereal Crop Research Unit, USDA-ARS, Fargo, ND, 58102, USA.
FAU - Steffenson, Brian J
AU  - Steffenson BJ
AD  - Department of Plant Pathology, University of Minnesota, Saint Paul, MN, 55108, 
      USA. bsteffen@umn.edu.
LA  - eng
PT  - Journal Article
DEP - 20160617
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Alleles
MH  - *Ascomycota
MH  - Chromosome Mapping
MH  - Disease Resistance/*genetics
MH  - Genetic Markers
MH  - Genotype
MH  - Haplotypes
MH  - Hordeum/*genetics/microbiology
MH  - Phenotype
MH  - Plant Breeding
MH  - Plant Diseases/*genetics/microbiology
MH  - Polymorphism, Single Nucleotide
MH  - *Quantitative Trait Loci
EDAT- 2016/06/19 06:00
MHDA- 2017/01/06 06:00
CRDT- 2016/06/19 06:00
PHST- 2015/12/09 00:00 [received]
PHST- 2016/06/06 00:00 [accepted]
PHST- 2016/06/19 06:00 [entrez]
PHST- 2016/06/19 06:00 [pubmed]
PHST- 2017/01/06 06:00 [medline]
AID - 10.1007/s00122-016-2742-y [pii]
AID - 10.1007/s00122-016-2742-y [doi]
PST - ppublish
SO  - Theor Appl Genet. 2016 Oct;129(10):1831-42. doi: 10.1007/s00122-016-2742-y. Epub 
      2016 Jun 17.