PMID- 27320013
OWN - NLM
STAT- MEDLINE
DCOM- 20170601
LR  - 20231213
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1861
IP  - 9 Pt A
DP  - 2016 Sep
TI  - Low-density lipoprotein upregulate SR-BI through Sp1 Ser702 phosphorylation in 
      hepatic cells.
PG  - 1066-1075
LID - S1388-1981(16)30153-6 [pii]
LID - 10.1016/j.bbalip.2016.06.001 [doi]
AB  - Scavenger receptor class B type I (SR-BI) is one of the key proteins in the 
      process of reverse cholesterol transport (RCT), and its major function is to 
      uptake high density lipoprotein (HDL) cholesterol from plasma into liver cells. 
      The regulation of SR-BI expression is important for controlling serum lipid 
      content and reducing the risks of cardiovascular diseases. Here we found that 
      SR-BI expression was significantly increased by LDL in vivo and in vitro, and the 
      transcription factor specific protein 1 (Sp1) plays a critical role in this 
      process. Results from co-immunoprecipitation experiments indicate that the 
      activation of SR-BI was associated with Sp1-recruited protein complexes in the 
      promoter region of SR-BI, where histone acetyltransferase p300 was recruited and 
      histone deacetylase HDAC1 was dismissed. As a result, histone acetylation 
      increased, leading to activation of SR-BI transcription. With further 
      investigation, we found that LDL phosphorylated Sp1 through ERK1/2 pathway, which 
      affected Sp1 protein complexes formation in SR-BI promoter. Using mass 
      spectrometry and site directed mutagenesis, a new Sp1 phosphorylation site Ser702 
      was defined to be associated with Sp1-HDAC1 interaction and may be important in 
      SR-BI activation, shedding light on the knowledge of delicate mechanism of 
      hepatic HDL receptor SR-BI gene modulation by LDL.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Yang, Fan
AU  - Yang F
AD  - Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute 
      of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, No. 1 Tiantan Xili, Beijing 100050, China.
FAU - Du, Yu
AU  - Du Y
AD  - Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute 
      of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, No. 1 Tiantan Xili, Beijing 100050, China.
FAU - Zhang, Jin
AU  - Zhang J
AD  - Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute 
      of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, No. 1 Tiantan Xili, Beijing 100050, China.
FAU - Jiang, Zhibo
AU  - Jiang Z
AD  - Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute 
      of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, No. 1 Tiantan Xili, Beijing 100050, China.
FAU - Wang, Li
AU  - Wang L
AD  - Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute 
      of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, No. 1 Tiantan Xili, Beijing 100050, China. Electronic address: 
      capricornkey@163.com.
FAU - Hong, Bin
AU  - Hong B
AD  - Key Laboratory of Biotechnology of Antibiotics of Ministry of Health, Institute 
      of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, No. 1 Tiantan Xili, Beijing 100050, China. Electronic address: 
      binhong69@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160615
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Apolipoproteins E)
RN  - 0 (Cholesterol, HDL)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (SCARB1 protein, human)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (SP1 protein, human)
RN  - EC 2.3.1.48 (p300-CBP Transcription Factors)
RN  - EC 2.3.1.48 (p300-CBP-associated factor)
RN  - EC 3.5.1.98 (HDAC1 protein, human)
RN  - EC 3.5.1.98 (Histone Deacetylase 1)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/*genetics
MH  - Cholesterol, HDL/genetics/metabolism
MH  - Gene Expression Regulation/genetics
MH  - Hep G2 Cells
MH  - Hepatocytes
MH  - Histone Deacetylase 1/*genetics/metabolism
MH  - Humans
MH  - Lipoproteins, LDL/genetics/metabolism
MH  - Liver/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Scavenger Receptors, Class B/*genetics/metabolism
MH  - Sp1 Transcription Factor/*biosynthesis/genetics
MH  - Transcriptional Activation/genetics
MH  - p300-CBP Transcription Factors/genetics/*metabolism
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Histone acetyltransferase/deacetylase
OT  - Low density lipoprotein (LDL)
OT  - Scavenger receptor class B type I (SR-BI)
OT  - Specific protein 1 (Sp1)
OT  - Transcriptional regulation
EDAT- 2016/06/21 06:00
MHDA- 2017/06/02 06:00
CRDT- 2016/06/21 06:00
PHST- 2016/01/15 00:00 [received]
PHST- 2016/05/04 00:00 [revised]
PHST- 2016/06/10 00:00 [accepted]
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2017/06/02 06:00 [medline]
AID - S1388-1981(16)30153-6 [pii]
AID - 10.1016/j.bbalip.2016.06.001 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2016 Sep;1861(9 Pt A):1066-1075. doi: 
      10.1016/j.bbalip.2016.06.001. Epub 2016 Jun 15.