PMID- 27320726
OWN - NLM
STAT- MEDLINE
DCOM- 20170127
LR  - 20220318
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 591
IP  - 2
DP  - 2016 Oct 15
TI  - Expressed miRNAs target feather related mRNAs involved in cell signaling, cell 
      adhesion and structure during chicken epidermal development.
PG  - 393-402
LID - S0378-1119(16)30490-5 [pii]
LID - 10.1016/j.gene.2016.06.027 [doi]
AB  - MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the 
      post-transcriptional level. Previous studies have shown that miRNA regulation 
      contributes to a diverse set of processes including cellular differentiation and 
      morphogenesis which leads to the creation of different cell types in 
      multicellular organisms and is thus key to animal development. Feathers are one 
      of the most distinctive features of extant birds and are important for multiple 
      functions including flight, thermal regulation, and sexual selection. However, 
      the role of miRNAs in feather development has been woefully understudied despite 
      the identification of cell signaling pathways, cell adhesion molecules and 
      structural genes involved in feather development. In this study, we performed a 
      microarray experiment comparing the expression of miRNAs and mRNAs among three 
      embryonic stages of development and two tissues (scutate scale and feather) of 
      the chicken. We combined this expression data with miRNA target prediction tools 
      and a curated list of feather related genes to produce a set of 19 miRNA-mRNA 
      duplexes. These targeted mRNAs have been previously identified as important cell 
      signaling and cell adhesion genes as well as structural genes involved in feather 
      and scale morphogenesis. Interestingly, the miRNA target site of the cell 
      signaling pathway gene, Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3), is 
      unique to birds indicating a novel role in Aves. The identified miRNA target site 
      of the cell adhesion gene, Tenascin C (TNC), is only found in specific chicken 
      TNC splice variants that are differentially expressed in developing scutate scale 
      and feather tissue indicating an important role of miRNA regulation in epidermal 
      differentiation. Additionally, we found that beta-keratins, a major structural 
      component of avian and reptilian epidermal appendages, are targeted by multiple 
      miRNA genes. In conclusion, our work provides quantitative expression data on 
      miRNAs and mRNAs during feather and scale development and has produced a highly 
      diverse, but manageable list of miRNA-mRNA duplexes for future validation 
      experiments.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Bao, Weier
AU  - Bao W
AD  - Department of Biological Sciences, University of South Carolina, Columbia, SC 
      29208, USA. Electronic address: bao2@email.sc.edu.
FAU - Greenwold, Matthew J
AU  - Greenwold MJ
AD  - Department of Biological Sciences, University of South Carolina, Columbia, SC 
      29208, USA. Electronic address: greenwold@biol.sc.edu.
FAU - Sawyer, Roger H
AU  - Sawyer RH
AD  - Department of Biological Sciences, University of South Carolina, Columbia, SC 
      29208, USA. Electronic address: rhsawyer@mailbox.sc.edu.
LA  - eng
GR  - P20 GM103499/GM/NIGMS NIH HHS/United States
GR  - P20 RR016461/RR/NCRR NIH HHS/United States
PT  - Journal Article
DEP - 20160615
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tenascin)
SB  - IM
MH  - Animals
MH  - *Cell Adhesion
MH  - Chickens
MH  - Epidermal Cells
MH  - Epidermis/growth & development/*metabolism
MH  - Evolution, Molecular
MH  - Feathers/*metabolism
MH  - MicroRNAs/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - *Signal Transduction
MH  - Tenascin/metabolism
MH  - Tissue Array Analysis
PMC - PMC5093910
MID - NIHMS798640
OTO - NOTNLM
OT  - ALDH1A3 and FGF20
OT  - Chicken
OT  - Evolution
OT  - Feather
OT  - Genome
OT  - MicroRNA
OT  - Scale
OT  - Tenascin C
OT  - beta-Keratin
EDAT- 2016/06/21 06:00
MHDA- 2017/01/28 06:00
PMCR- 2017/10/15
CRDT- 2016/06/21 06:00
PHST- 2016/01/10 00:00 [received]
PHST- 2016/06/03 00:00 [revised]
PHST- 2016/06/13 00:00 [accepted]
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2017/01/28 06:00 [medline]
PHST- 2017/10/15 00:00 [pmc-release]
AID - S0378-1119(16)30490-5 [pii]
AID - 10.1016/j.gene.2016.06.027 [doi]
PST - ppublish
SO  - Gene. 2016 Oct 15;591(2):393-402. doi: 10.1016/j.gene.2016.06.027. Epub 2016 Jun 
      15.