PMID- 27320800
OWN - NLM
STAT- MEDLINE
DCOM- 20170314
LR  - 20190606
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 22
DP  - 2016 Jun 20
TI  - Expression and Significance of High-Mobility Group Protein B1 (HMGB1) and the 
      Receptor for Advanced Glycation End-Product (RAGE) in Knee Osteoarthritis.
PG  - 2105-12
AB  - BACKGROUND This study was performed with the aim to explore the expression of 
      high-mobility group protein B1 (HMGB1) and the receptor for advanced glycation 
      end-product (RAGE) in knee osteoarthritis (KOA) and its clinical significance. 
      MATERIAL AND METHODS A total of 108 synovial tissues selected from KOA patients 
      were included in the experimental group. Seventy-five synovial tissues of knee 
      joints, selected from patients who were clinically and pathologically confirmed 
      without joint lesion, were included in the control group. The mRNA and protein 
      expressions of HMGB1 and RAGE were determined by using RT-PCR and 
      immunohistochemistry, respectively. Western blotting was used for measuring 
      relative protein expression. An ROC curve was drawn to evaluate the diagnostic 
      value of HMGB1 and RAGE for KOA. RESULTS The positive cell number and positive 
      expression intensity of HMGB1 and RAGE in synovial tissue was higher in the 
      experimental group than in the control group. PI for HMGB1 and RAGE expression in 
      KOA patients was positively correlated with clinical classification of X-ray 
      films (P<0.05). HMGB1 and RAGE mRNA expressions, as well as relative protein 
      expression of HMGB1 and RAGE in synovial tissue, were higher in the experimental 
      group than in the control group (all P<0.05). The sensitivity of HMGB1 protein, 
      RAGE protein, HMGB1 mRNA, and RAGE mRNA were 76.9%, 64.8%, 86.1%, and 64.8%, 
      respectively; and the specificity was 100%, 96%, 74.7%, and 80%, respectively. 
      CONCLUSIONS The protein and mRNA expressions of HMGB1 and RAGE are both increased 
      in KOA patients, suggesting that they are involved in KOA.
FAU - Sun, Xue-Hui
AU  - Sun XH
AD  - Department of Rheumatology, Yuhuangding Hospital Affiliated to Qingdao 
      University, Yantai, Shandong, China (mainland).
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Rheumatology, Yuhuangding Hospital Affiliated to Qingdao 
      University, Yantai, Shandong, China (mainland).
FAU - Han, Yun
AU  - Han Y
AD  - Department of Anesthesiology, Yuhuangding Hospital Affiliated to Qingdao 
      University, Yantai, Shandong, China (mainland).
FAU - Wang, Jian
AU  - Wang J
AD  - Department of Rheumatology, Yuhuangding Hospital Affiliated to Qingdao 
      University, Yantai, Shandong, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20160620
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.22 (MOK protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Neoplasm/*biosynthesis/genetics/metabolism
MH  - Female
MH  - HMGB1 Protein/*biosynthesis/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Joint Capsule/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Mitogen-Activated Protein Kinases/*biosynthesis/genetics/metabolism
MH  - Osteoarthritis, Knee/genetics/*metabolism/pathology
MH  - RNA, Messenger/biosynthesis/genetics/metabolism
MH  - ROC Curve
MH  - Transcriptome
PMC - PMC4918532
EDAT- 2016/06/21 06:00
MHDA- 2017/03/16 06:00
PMCR- 2016/06/20
CRDT- 2016/06/21 06:00
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2017/03/16 06:00 [medline]
PHST- 2016/06/20 00:00 [pmc-release]
AID - 895689 [pii]
AID - 10.12659/msm.895689 [doi]
PST - epublish
SO  - Med Sci Monit. 2016 Jun 20;22:2105-12. doi: 10.12659/msm.895689.