PMID- 27321406
OWN - NLM
STAT- MEDLINE
DCOM- 20170405
LR  - 20170405
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 51
IP  - 4
DP  - 2016 Aug
TI  - Comparison of DNA Fragmentation Assay in Frozen-Thawed Cat Epididymal Sperm.
PG  - 618-22
LID - 10.1111/rda.12710 [doi]
AB  - DNA fragmentation of frozen-thawed feline epididymal sperm from corpus and cauda 
      regions was evaluated by three different techniques. The DNA fragmentation index 
      (DFI) was compared between techniques: the sperm chromatin structural assay 
      (SCSA((R)) ), acridine orange staining techniques (AOT) and the sperm chromatin 
      dispersion (SCD). There were significant differences in DFI among the techniques 
      (p < 0.05) with no correlations. Only DFI values obtained from SCD revealed a 
      significantly higher DFI in corpus compared with cauda spermatozoa (p < 0.05). 
      The discrepancy between techniques might be due to the sensitivity of each 
      technique, differences in severity of DNA damaged that can be detected. The 
      difference in DFI between epididymal regions from SCD technique might indicate 
      different maturational stages of spermatozoa, with less chromatin condensation of 
      spermatozoa in corpus compared with cauda epididymis.
CI  - (c) 2016 Blackwell Verlag GmbH.
FAU - Kunkitti, P
AU  - Kunkitti P
AD  - Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary 
      Medicine and Animal Science, Swedish University of Agricultural Sciences, 
      Uppsala, Sweden.
AD  - Department of Surgery and Theriogenology, Faculty of Veterinary Medicine, Khon 
      Kaen University, Khon Kaen, Thailand.
FAU - Sjodahl, A
AU  - Sjodahl A
AD  - Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary 
      Medicine and Animal Science, Swedish University of Agricultural Sciences, 
      Uppsala, Sweden.
FAU - Bergqvist, A-S
AU  - Bergqvist AS
AD  - Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary 
      Medicine and Animal Science, Swedish University of Agricultural Sciences, 
      Uppsala, Sweden.
FAU - Johannisson, A
AU  - Johannisson A
AD  - Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary 
      Medicine and Animal Science, Swedish University of Agricultural Sciences, 
      Uppsala, Sweden.
FAU - Axner, E
AU  - Axner E
AD  - Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary 
      Medicine and Animal Science, Swedish University of Agricultural Sciences, 
      Uppsala, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20160620
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
SB  - IM
MH  - Animals
MH  - *Cats
MH  - Cryopreservation/*veterinary
MH  - *DNA Fragmentation
MH  - Epididymis/*cytology
MH  - Freezing
MH  - Male
MH  - Semen Preservation/*veterinary
MH  - Spermatozoa/*physiology
EDAT- 2016/06/21 06:00
MHDA- 2017/04/06 06:00
CRDT- 2016/06/21 06:00
PHST- 2015/12/11 00:00 [received]
PHST- 2016/04/25 00:00 [accepted]
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2017/04/06 06:00 [medline]
AID - 10.1111/rda.12710 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2016 Aug;51(4):618-22. doi: 10.1111/rda.12710. Epub 2016 Jun 
      20.