PMID- 27321991
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR  - 20220410
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jun 20
TI  - Alpinetin attenuates inflammatory responses by suppressing TLR4 and NLRP3 
      signaling pathways in DSS-induced acute colitis.
PG  - 28370
LID - 10.1038/srep28370 [doi]
LID - 28370
AB  - Alpinetin, a composition of Alpinia katsumadai Hayata, has been reported to have 
      a number of biological properties, such as antibacterial, antitumor and other 
      important therapeutic activities. However, the effect of alpinetin on 
      inflammatory bowel disease (IBD) has not yet been reported. The purpose of this 
      study was to investigate the anti-inflammatory effect and mechanism of alpinetin 
      on dextran sulfate sodium (DSS)-induced colitis in mice. In vivo, DSS-induced 
      mice colitis model was established by giving mice drinking water containing 5% 
      (w/v) DSS for 7 days. Alpinetin (25, 50 and 100 mg/kg) were administered once a 
      day by intraperitoneal injection 3 days before DSS treatment. In vitro, phorbol 
      myristate acetate (PMA)-differentiated monocytic THP-1 macrophages were treated 
      with alpinetin and stimulated by lipopolysaccharide (LPS). The results showed 
      that alpinetin significantly attenuated diarrhea, colonic shortening, 
      histological injury, myeloperoxidase (MPO) activity and the expressions of tumor 
      necrosis factor (TNF-alpha) and interleukin (IL-1beta) production in mice. In vitro, 
      alpinetin markedly inhibited LPS-induced TNF-alpha and IL-1beta production, as well as 
      Toll-like receptor 4 (TLR4) mediated nuclear transcription factor-kappaB (NF-kappaB) 
      and NOD-like receptor protein 3 (NLRP3) inflammasome activation. In conclusion, 
      this study demonstrated that alpinetin had protective effects on DSS-induced 
      colitis and may be a promising therapeutic reagent for colitis treatment.
FAU - He, Xuexiu
AU  - He X
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
FAU - Wei, Zhengkai
AU  - Wei Z
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
FAU - Wang, Jingjing
AU  - Wang J
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
FAU - Kou, Jinhua
AU  - Kou J
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
FAU - Liu, Weijian
AU  - Liu W
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
FAU - Fu, Yunhe
AU  - Fu Y
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
FAU - Yang, Zhengtao
AU  - Yang Z
AD  - College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160620
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Flavanones)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (alpinetin)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Colitis/chemically induced/*immunology
MH  - Dextran Sulfate
MH  - Female
MH  - Flavanones/*pharmacology
MH  - Humans
MH  - Inflammasomes/metabolism
MH  - Inflammation/*drug therapy
MH  - Injections, Intraperitoneal
MH  - Interleukin-1beta/metabolism
MH  - Lipopolysaccharides
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - NF-kappa B/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/*metabolism
MH  - Peroxidase/metabolism
MH  - *Signal Transduction
MH  - THP-1 Cells/cytology
MH  - Tetradecanoylphorbol Acetate
MH  - Toll-Like Receptor 4/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC4913257
EDAT- 2016/06/21 06:00
MHDA- 2018/06/05 06:00
PMCR- 2016/06/20
CRDT- 2016/06/21 06:00
PHST- 2015/11/15 00:00 [received]
PHST- 2016/06/03 00:00 [accepted]
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
PHST- 2016/06/20 00:00 [pmc-release]
AID - srep28370 [pii]
AID - 10.1038/srep28370 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jun 20;6:28370. doi: 10.1038/srep28370.