PMID- 27322053
OWN - NLM
STAT- MEDLINE
DCOM- 20170210
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 39
IP  - 1
DP  - 2016
TI  - Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in 
      a Rat Model.
PG  - 33-46
LID - 10.1159/000445603 [doi]
AB  - BACKGROUND: Intra-abdominal adhesions are a very common complication following 
      abdominal surgery. Our previous studies have demonstrated that the inhibition of 
      inflammation at the sites of peritoneal injury can prevent the formation of 
      intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of 
      anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the 
      formation of intra-abdominal adhesions after surgery. The aim of this study was 
      to investigate the effect of resveratrol on intra-abdominal adhesion prevention 
      in a rat model with surgery-induced peritoneal adhesions. MATERIALS AND METHODS: 
      The cecum wall and its opposite parietal peritoneum were abraded following 
      laparotomy to induce intra-abdominal adhesion formation. Varying doses of 
      resveratrol were administered to the animals. On the eighth day after surgery, 
      the adhesion score was assessed using a visual scoring system. Picrosirius red 
      staining and a hydroxyproline assay were used to assess the amount of collagen 
      deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), 
      tumor necrosis factor (TNF-alpha), and transforming growth factor beta-1 (TGF-beta1) 
      were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting 
      was performed to determine the protein expression of TGF-beta1, fibrinogen, and 
      alpha-smooth muscle actin (alpha-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR 
      was performed to quantify the mRNA expression of TGF-beta1, fibrinogen, and alpha-SMA. 
      RESULTS: Resveratrol significantly reduced intra-abdominal adhesion formation and 
      fibrin deposition in the rat model. Furthermore, resveratrol significantly 
      reduced the serum levels of IL-6, TNF-alpha, and TGF-beta1. The protein and mRNA 
      expression of TGF-beta1, fibrinogen, and alpha-SMA in the rat peritoneum and adhesion 
      tissues were also down-regulated due to resveratrol intervention. CONCLUSION: 
      Resveratrol can effectively prevent the formation of postoperative 
      intra-abdominal adhesions in a rat model. This effect may be related to the 
      suppression of inflammatory cytokine expression in the injured peritoneum by 
      resveratrol. This study suggests that resveratrol may be a new and effective 
      anti-adhesive agent that is worthy of further study and has potential application 
      value.
CI  - (c) 2016 The Author(s) Published by S. Karger AG, Basel.
FAU - Wei, Guangbing
AU  - Wei G
AD  - Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Chen, Xin
AU  - Chen X
FAU - Wang, Guanghui
AU  - Wang G
FAU - Fan, Lin
AU  - Fan L
FAU - Wang, Kang
AU  - Wang K
FAU - Li, Xuqi
AU  - Li X
LA  - eng
PT  - Journal Article
DEP - 20160620
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Actins)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Interleukin-6)
RN  - 0 (Stilbenes)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (smooth muscle actin, rat)
RN  - 9001-32-5 (Fibrinogen)
RN  - 9007-34-5 (Collagen)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Abdomen/*surgery
MH  - Actins/genetics/metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Blotting, Western
MH  - Collagen/metabolism
MH  - *Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibrinogen/genetics/metabolism
MH  - Interleukin-6/blood/metabolism
MH  - Male
MH  - Peritoneum/metabolism/pathology
MH  - Postoperative Complications/etiology/metabolism/*prevention & control
MH  - Rats, Sprague-Dawley
MH  - Resveratrol
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stilbenes/*pharmacology
MH  - Tissue Adhesions/etiology/metabolism/*prevention & control
MH  - Transforming Growth Factor beta1/blood/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/blood/metabolism
EDAT- 2016/06/21 06:00
MHDA- 2017/02/12 06:00
CRDT- 2016/06/21 06:00
PHST- 2016/05/09 00:00 [accepted]
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2017/02/12 06:00 [medline]
AID - 000445603 [pii]
AID - 10.1159/000445603 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2016;39(1):33-46. doi: 10.1159/000445603. Epub 2016 Jun 20.