PMID- 27322965
OWN - NLM
STAT- MEDLINE
DCOM- 20170322
LR  - 20231213
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 19
IP  - 7
DP  - 2016 Jul
TI  - The Soy Peptide Phe-Leu-Val Reduces TNFalpha-Induced Inflammatory Response and 
      Insulin Resistance in Adipocytes.
PG  - 678-85
LID - 10.1089/jmf.2016.3685 [doi]
AB  - Obesity-induced adipose inflammation plays a crucial role in the development of 
      obesity-induced metabolic disorders such as insulin resistance and type 2 
      diabetes. In the presence of obesity, hypertrophic adipocytes release 
      inflammatory mediators, including tumor necrosis factor-alpha (TNFalpha) and monocyte 
      chemoattractant protein-1 (MCP-1), which enhance the recruitment and activation 
      of macrophages, and in turn augment adipose inflammation. We demonstrate that the 
      soy peptide Phe-Leu-Val (FLV) reduces inflammatory responses and insulin 
      resistance in mature adipocytes. Specifically, the soy peptide FLV inhibits the 
      release of inflammatory cytokines (TNFalpha, MCP-1, and IL-6) from both 
      TNFalpha-stimulated adipocytes and cocultured adipocytes/macrophages. This inhibition 
      is mediated by the inactivation of the inflammatory signaling molecules c-Jun 
      N-terminal kinase (JNK) and IkappaB kinase (IKK), and the downregulation of IkappaBalpha in 
      the adipocytes. In addition, soy peptide FLV enhances insulin responsiveness and 
      increases glucose uptake in adipocytes. More importantly, we, for the first time, 
      found that adipocytes express peptide transporter 2 (PepT2) protein, and the 
      beneficial action of the soy peptide FLV was disrupted by the peptide transporter 
      inhibitor GlySar. These findings suggest that soy peptide FLV is transported into 
      adipocytes by PepT2 and then downregulates TNFalpha-induced inflammatory signaling, 
      thereby increasing insulin responsiveness in the cells. The soy peptide FLV, 
      therefore, has the potential to prevent obesity-induced adipose inflammation and 
      insulin resistance.
FAU - Kwak, Su-Jin
AU  - Kwak SJ
AD  - 1 Department of Food Science and Nutrition, University of Ulsan , Ulsan, Korea.
FAU - Kim, Chu-Sook
AU  - Kim CS
AD  - 1 Department of Food Science and Nutrition, University of Ulsan , Ulsan, Korea.
FAU - Choi, Myung-Sook
AU  - Choi MS
AD  - 2 Department of Food Science and Nutrition, Center for Food and Nutritional 
      Genomics Research, Kyungpook National University , Daegu, Korea.
FAU - Park, Taesun
AU  - Park T
AD  - 3 Department of Food and Nutrition, Yonsei University , Seoul, Korea.
FAU - Sung, Mi-Kyung
AU  - Sung MK
AD  - 4 Department of Food and Nutrition, Sookmyung Women's University , Seoul, Korea.
FAU - Yun, Jong Won
AU  - Yun JW
AD  - 5 Department of Biotechnology, Daegu University , Gyeongbuk, Korea.
FAU - Yoo, Hoon
AU  - Yoo H
AD  - 6 Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chosun 
      University , Gwangju, Korea.
FAU - Mine, Yoshinori
AU  - Mine Y
AD  - 7 Department of Food Science, University of Guelph , Guelph, ON, Canada .
FAU - Yu, Rina
AU  - Yu R
AD  - 1 Department of Food Science and Nutrition, University of Ulsan , Ulsan, Korea.
LA  - eng
PT  - Journal Article
DEP - 20160620
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Oligopeptides)
RN  - 0 (Symporters)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (hydrogen-coupled oligopeptide transporter PepT2)
RN  - 0 (phenylalanyl-leucyl-valine)
SB  - IM
MH  - Adipocytes/*drug effects/metabolism
MH  - *Anti-Inflammatory Agents
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/metabolism
MH  - Humans
MH  - Inflammation/prevention & control
MH  - *Insulin Resistance
MH  - Obesity/metabolism
MH  - Oligopeptides/*pharmacology
MH  - Signal Transduction/drug effects
MH  - Glycine max/*chemistry
MH  - Symporters/antagonists & inhibitors/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism/*pharmacology
OTO - NOTNLM
OT  - adipocytes
OT  - inflammation
OT  - insulin resistance
OT  - obesity
OT  - soy peptide
EDAT- 2016/06/21 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/06/21 06:00
PHST- 2016/06/21 06:00 [entrez]
PHST- 2016/06/21 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
AID - 10.1089/jmf.2016.3685 [doi]
PST - ppublish
SO  - J Med Food. 2016 Jul;19(7):678-85. doi: 10.1089/jmf.2016.3685. Epub 2016 Jun 20.