PMID- 27327408 OWN - NLM STAT- MEDLINE DCOM- 20181016 LR - 20181016 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) VI - 8 IP - 27 DP - 2016 Jul 13 TI - From Solution to Biointerface: Graphene Self-Assemblies of Varying Lateral Sizes and Surface Properties for Biofilm Control and Osteodifferentiation. PG - 17151-65 LID - 10.1021/acsami.6b05198 [doi] AB - Bringing multifunctional graphene out of solution through facile self-assembly to form 2D surface nanostructures, with control over the lateral size and surface properties, would be an intriguing accomplishment, especially in biomedical fields where biointerfaces with functional diversity are in high demand. Guided by this goal, in this work, we built such graphene-based self-assemblies on orthopedic titanium, attempting to selectively regulate bacterial activities and osteoblastic functions, which are both crucial in bone regeneration. Briefly, large-area graphene oxide (GO) sheets and functionalized reduced GO (rGO) micro-/nanosheets were self-assembled spontaneously and controllably onto solid Ti, through an evaporation-assisted electrostatic assembly process and a mussel-inspired one-pot assembly process, respectively. The resultant layers were characterized in terms of topological structure, chemical composition, hydrophilicity, and protein adsorption properties. The antibacterial efficacies of the assemblies were examined by challenging them with pathogenic Staphylococcus aureus (S. aureus) bacteria that produce biofilms, whereby around 50% antiadhesion effects and considerable antibiofilm activities were observed for both layer types but through dissimilar modes of action. Their cytocompatibility and osteogenic potential were also investigated. Interfaced with MC3T3-E1 cells, the functionalized rGO sheets evoked better cell adhesion and growth than GO sheets, whereas the latter elicited higher osteodifferentiation activity throughout a 28-day in vitro culture. In this work, we showed that it is technically possible to construct graphene interface layers of varying lateral dimensions and surface properties and confirmed the concept of using the obtained assemblies to address the two major challenges facing orthopedic clinics. In addition, we determined fundamental implications for understanding the surface-biology relationship of graphene biomaterials, in efforts to better design and more safely use them for future biomedicine. FAU - Jia, Zhaojun AU - Jia Z AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Shi, Yuying AU - Shi Y AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Xiong, Pan AU - Xiong P AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Zhou, Wenhao AU - Zhou W AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Cheng, Yan AU - Cheng Y AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Zheng, Yufeng AU - Zheng Y AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Xi, Tingfei AU - Xi T AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. FAU - Wei, Shicheng AU - Wei S AD - Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, double daggerDepartment of Advanced Materials and Nanotechnology, College of Engineering, and section signDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University , Beijing 100871, China. LA - eng PT - Journal Article DEP - 20160628 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 RN - 0 (Oxides) RN - 7782-42-5 (Graphite) SB - IM MH - Animals MH - Biofilms MH - Cell Differentiation MH - Cell Line MH - Graphite/*chemistry MH - Mice MH - Oxides MH - Staphylococcus aureus MH - Surface Properties OTO - NOTNLM OT - antibacterial OT - cytotoxicity OT - graphene nanomaterials OT - osteogenic OT - poly(dopamine) OT - self-assembly EDAT- 2016/06/22 06:00 MHDA- 2018/10/17 06:00 CRDT- 2016/06/22 06:00 PHST- 2016/06/22 06:00 [entrez] PHST- 2016/06/22 06:00 [pubmed] PHST- 2018/10/17 06:00 [medline] AID - 10.1021/acsami.6b05198 [doi] PST - ppublish SO - ACS Appl Mater Interfaces. 2016 Jul 13;8(27):17151-65. doi: 10.1021/acsami.6b05198. Epub 2016 Jun 28.