PMID- 27328025
OWN - NLM
STAT- MEDLINE
DCOM- 20180716
LR  - 20180716
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 29
IP  - 7
DP  - 2017 Jul
TI  - Subfertility in androgen-insensitive female mice is rescued by transgenic FSH.
PG  - 1426-1434
LID - 10.1071/RD16022 [doi]
AB  - Androgens synergise with FSH in female reproduction but the nature of their 
      interaction in ovarian function and fertility is not clear. In the present study, 
      we investigated this interaction, notably whether higher endogenous FSH can 
      overcome defective androgen actions in androgen receptor (AR)-knockout (ARKO) 
      mice. We generated and investigated the reproductive function of mutant mice 
      exhibiting AR resistance with or without expression of human transgenic FSH 
      (Tg-FSH). On the background of inactivated AR signalling, which alone resulted in 
      irregular oestrous cycles and reduced pups per litter, ovulation rates and antral 
      follicle health, Tg-FSH expression restored follicle health, ovulation rates and 
      litter size to wild-type levels. However, Tg-FSH was only able to partially 
      rectify the abnormal oestrous cycles observed in ARKO females. Hence, elevated 
      endogenous FSH rescued the intraovarian defects, and partially rescued the 
      extraovarian defects due to androgen insensitivity. In addition, the observed 
      increase in litter size in Tg-FSH females was not observed in the presence of AR 
      signalling inactivation. In summary, the findings of the present study reveal 
      that FSH can rescue impaired female fertility and ovarian function due to 
      androgen insensitivity in female ARKO mice by maintaining follicle health and 
      ovulation rates, and thereby optimal female fertility.
FAU - Walters, K A
AU  - Walters KA
AD  - ANZAC Research Institute, Andrology Laboratory, University of Sydney, Concord 
      Hospital, Hospital Road, Concord, NSW 2139, Australia.
FAU - Edwards, M C
AU  - Edwards MC
AD  - ANZAC Research Institute, Andrology Laboratory, University of Sydney, Concord 
      Hospital, Hospital Road, Concord, NSW 2139, Australia.
FAU - Jimenez, M
AU  - Jimenez M
AD  - ANZAC Research Institute, Andrology Laboratory, University of Sydney, Concord 
      Hospital, Hospital Road, Concord, NSW 2139, Australia.
FAU - Handelsman, D J
AU  - Handelsman DJ
AD  - ANZAC Research Institute, Andrology Laboratory, University of Sydney, Concord 
      Hospital, Hospital Road, Concord, NSW 2139, Australia.
FAU - Allan, C M
AU  - Allan CM
AD  - ANZAC Research Institute, Andrology Laboratory, University of Sydney, Concord 
      Hospital, Hospital Road, Concord, NSW 2139, Australia.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (AR protein, mouse)
RN  - 0 (Follicle Stimulating Hormone, Human)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Estrus
MH  - Female
MH  - Fertility/genetics/physiology
MH  - Follicle Stimulating Hormone, Human/*genetics/*physiology
MH  - Humans
MH  - Infertility, Female/genetics/physiopathology/*therapy
MH  - Litter Size
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Ovary/pathology/physiopathology
MH  - Pregnancy
MH  - Receptors, Androgen/*deficiency/genetics
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 2016/06/22 06:00
MHDA- 2018/07/17 06:00
CRDT- 2016/06/22 06:00
PHST- 2016/01/12 00:00 [received]
PHST- 2016/05/30 00:00 [accepted]
PHST- 2016/06/22 06:00 [pubmed]
PHST- 2018/07/17 06:00 [medline]
PHST- 2016/06/22 06:00 [entrez]
AID - RD16022 [pii]
AID - 10.1071/RD16022 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2017 Jul;29(7):1426-1434. doi: 10.1071/RD16022.