PMID- 27328268
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20221207
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 18
IP  - 11
DP  - 2016 Nov
TI  - Isolation of Bdellovibrio bacteriovorus from a tropical wastewater treatment 
      plant and predation of mixed species biofilms assembled by the native community 
      members.
PG  - 3923-3931
LID - 10.1111/1462-2920.13384 [doi]
AB  - It is reported here that a predatory bacterium belonging to the Genus 
      Bdellovibrio, was isolated from activated sludge at the Ulu Pandan Water 
      Reclamation Plant, Singapore. 16S rDNA gene sequencing analysis revealed that 
      this isolate was 99% identical to 'Bdellovibrio bacteriovorus strain Tiberius' 
      and hence is designated as 'Bdellovibrio bacteriovorus UP'. Using a novel 
      approach based on fluorescence in situ hybridization (FISH), a prey cell 
      density-dependent growth pattern of B. bacteriovorus UP was established. B. 
      bacteriovorus UP preyed upon a broad range of bacterial species (60 species) 
      isolated from the activated sludge. Except for Ochrobactrum anthropi, all 
      Gram-negative species were sensitive to predation by B. bacteriovorus UP 
      irrespective of the mode of growth (planktonic or biofilm). Similarly, the 
      predation-sensitive species were not protected by the predation-resistant 
      species, O. anthropi, as determined in multiple dual-species planktonic and 
      biofilm consortia. Given the broad prey spectrum, B. bacteriovorus UP may impact 
      functional community members, which are largely members of the Proteobacteria. 
      Thus, these results provide an important insight to the role of predatory 
      bacteria in shaping of community structure and function in both natural and 
      engineered ecosystems.
CI  - (c) 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Feng, Shugeng
AU  - Feng S
AD  - The Singapore Centre for Environmental Life Sciences Engineering, Nanyang 
      Technological University, Singapore.
FAU - Tan, Chuan Hao
AU  - Tan CH
AD  - The Singapore Centre for Environmental Life Sciences Engineering, Nanyang 
      Technological University, Singapore.
AD  - The School of Materials Science & Engineering, Nanyang Technological University, 
      Singapore.
FAU - Cohen, Yehuda
AU  - Cohen Y
AD  - The Singapore Centre for Environmental Life Sciences Engineering, Nanyang 
      Technological University, Singapore.
FAU - Rice, Scott A
AU  - Rice SA
AD  - The Singapore Centre for Environmental Life Sciences Engineering, Nanyang 
      Technological University, Singapore.
AD  - The School of Biological Sciences, Nanyang Technological University, Singapore.
LA  - eng
PT  - Journal Article
DEP - 20160708
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Waste Water)
SB  - IM
MH  - Bdellovibrio bacteriovorus/genetics/*isolation & purification/*physiology
MH  - Biodegradation, Environmental
MH  - Biodiversity
MH  - *Biofilms
MH  - In Situ Hybridization, Fluorescence
MH  - Wastewater/*microbiology
MH  - Water Purification/instrumentation
EDAT- 2016/06/22 06:00
MHDA- 2017/07/27 06:00
CRDT- 2016/06/22 06:00
PHST- 2016/06/22 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/06/22 06:00 [entrez]
AID - 10.1111/1462-2920.13384 [doi]
PST - ppublish
SO  - Environ Microbiol. 2016 Nov;18(11):3923-3931. doi: 10.1111/1462-2920.13384. Epub 
      2016 Jul 8.