PMID- 27328657
OWN - NLM
STAT- MEDLINE
DCOM- 20180419
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jun 22
TI  - Functional characterization of three trehalase genes regulating the chitin 
      metabolism pathway in rice brown planthopper using RNA interference.
PG  - 27841
LID - 10.1038/srep27841 [doi]
LID - 27841
AB  - RNA interference (RNAi) is an effective gene-silencing tool, and double stranded 
      RNA (dsRNA) is considered a powerful strategy for gene function studies in 
      insects. In the present study, we aimed to investigate the function of trehalase 
      (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper 
      Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate 
      their regulating roles in chitin synthesis by injecting larvae with dsRNA. The 
      results showed that TRE1 and TRE2 had compensatory function, and the expression 
      of each increased when the other was silenced. The total rate of insects with 
      phenotypic deformities ranged from 19.83 to 24.36% after dsTRE injection, whereas 
      the mortality rate ranged from 14.16 to 31.78%. The mRNA levels of genes involved 
      in the chitin metabolism pathway in RNA-Seq and DGEP, namely hexokinase (HK), 
      glucose-6-phosphate isomerase (G6PI) and chitinase (Cht), decreased significantly 
      at 72 h after single dsTREs injection, whereas two transcripts of chitin synthase 
      (CHS) genes decreased at 72 h after dsTRE1-1 and dsTREs injection. These results 
      demonstrated that TRE silencing could affect the regulation of chitin 
      biosynthesis and degradation, causing moulting deformities. Therefore, expression 
      inhibitors of TREs might be effective tools for the control of planthoppers in 
      rice.
FAU - Zhao, Lina
AU  - Zhao L
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
FAU - Yang, Mengmeng
AU  - Yang M
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
FAU - Shen, Qida
AU  - Shen Q
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
FAU - Liu, Xiaojun
AU  - Liu X
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
FAU - Shi, Zuokun
AU  - Shi Z
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
FAU - Wang, Shigui
AU  - Wang S
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
FAU - Tang, Bin
AU  - Tang B
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160622
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Insect Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 1398-61-4 (Chitin)
RN  - EC 3.2.1.28 (Trehalase)
SB  - IM
MH  - Animals
MH  - Chitin/*biosynthesis
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Regulatory Networks
MH  - Hemiptera/*enzymology/genetics/*growth & development
MH  - Insect Proteins/genetics/metabolism
MH  - *Metabolic Networks and Pathways
MH  - Oryza/parasitology
MH  - RNA Interference
MH  - RNA, Double-Stranded/administration & dosage
MH  - Sequence Analysis, RNA
MH  - Trehalase/*genetics/metabolism
PMC - PMC4916506
EDAT- 2016/06/23 06:00
MHDA- 2018/04/20 06:00
PMCR- 2016/06/22
CRDT- 2016/06/23 06:00
PHST- 2016/02/01 00:00 [received]
PHST- 2016/05/25 00:00 [accepted]
PHST- 2016/06/23 06:00 [entrez]
PHST- 2016/06/23 06:00 [pubmed]
PHST- 2018/04/20 06:00 [medline]
PHST- 2016/06/22 00:00 [pmc-release]
AID - srep27841 [pii]
AID - 10.1038/srep27841 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jun 22;6:27841. doi: 10.1038/srep27841.