PMID- 27330522
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1573-3882 (Print)
IS  - 1573-3890 (Electronic)
IS  - 1573-3882 (Linking)
VI  - 12
DP  - 2016
TI  - Metabolic fingerprints of human primary endothelial and fibroblast cells.
PG  - 92
LID - 92
AB  - INTRODUCTION: Human primary cells originating from different locations within the 
      body could differ greatly in their metabolic phenotypes, influencing both how 
      they act during physiological/pathological processes and how 
      susceptible/resistant they are to a variety of disease risk factors. A novel way 
      to monitor cellular metabolism is through cell energetics assays, so we explored 
      this approach with human primary cell types, as models of sclerotic disorders. 
      OBJECTIVES: In order to better understand pathophysiological processes at the 
      cellular level, our goals were to measure metabolic pathway activities of 
      endothelial cells and fibroblasts, and determine their metabolic phenotype 
      profiles. METHODS: Biolog Phenotype MicroArray technology was used for the first 
      time to characterize metabolic phenotypes of diverse primary cells. These 
      colorimetric assays enable detection of utilization of 367 specific biochemical 
      substrates by human endothelial cells from the coronary artery (HCAEC), umbilical 
      vein (HUVEC) and normal, healthy lung fibroblasts (NHLF). RESULTS: Adenosine, 
      inosine, d-mannose and dextrin were strongly utilized by all three cell types, 
      comparable to glucose. Substrates metabolized solely by HCAEC were mannan, 
      pectin, gelatin and prevalently tricarballylic acid. HUVEC did not show any 
      uniquely metabolized substrates whereas NHLF exhibited strong utilization of 
      sugars and carboxylic acids along with amino acids and peptides. CONCLUSION: 
      Taken together, we show for the first time that this simple energetics assay 
      platform enables metabolic characterization of primary cells and that each of the 
      three human cell types examined gives a unique and distinguishable profile.
FAU - Zigon, Polona
AU  - Zigon P
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Mrak-Poljsak, Katjusa
AU  - Mrak-Poljsak K
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Lakota, Katja
AU  - Lakota K
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Tercelj, Matic
AU  - Tercelj M
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Cucnik, Sasa
AU  - Cucnik S
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia ; Faculty of Pharmacy, Chair of Clinical Biochemistry, 
      University of Ljubljana, Ljubljana, Slovenia.
FAU - Tomsic, Matija
AU  - Tomsic M
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Sodin-Semrl, Snezna
AU  - Sodin-Semrl S
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia ; Faculty of Mathematics, Natural Sciences and 
      Information Technology, University of Primorska, Koper, Slovenia.
LA  - eng
PT  - Journal Article
DEP - 20160407
PL  - United States
TA  - Metabolomics
JT  - Metabolomics : Official journal of the Metabolomic Society
JID - 101274889
PMC - PMC4887525
OTO - NOTNLM
OT  - Cellular metabolism
OT  - Human primary cells
OT  - OmniLog
OT  - Phenomics
OT  - Phenotype MicroArrays
EDAT- 2016/06/23 06:00
MHDA- 2016/06/23 06:00
PMCR- 2016/04/07
CRDT- 2016/06/23 06:00
PHST- 2015/11/05 00:00 [received]
PHST- 2016/03/18 00:00 [accepted]
PHST- 2016/06/23 06:00 [entrez]
PHST- 2016/06/23 06:00 [pubmed]
PHST- 2016/06/23 06:00 [medline]
PHST- 2016/04/07 00:00 [pmc-release]
AID - 1024 [pii]
AID - 10.1007/s11306-016-1024-7 [doi]
PST - ppublish
SO  - Metabolomics. 2016;12:92. doi: 10.1007/s11306-016-1024-7. Epub 2016 Apr 7.