PMID- 27330564
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160622
LR  - 20201001
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 9
DP  - 2016
TI  - Comparative analysis of individual chromosome involvement in micronuclei induced 
      by mitomycin C and bleomycin in human leukocytes.
PG  - 49
LID - 10.1186/s13039-016-0258-4 [doi]
LID - 49
AB  - BACKGROUND: Micronucleus (MN) assay is a well standardized approach for 
      evaluation of clastogenic/aneugenic effects of mutagens. Fluorescence in situ 
      hybridization (FISH) is successfully used to characterize the chromosomal content 
      of MN. However, the relationships between nuclear positioning, length, and gene 
      density of individual chromosomes and their involvement in MN induced by 
      different mutagens have not been clearly defined. RESULTS: Chromosomal content of 
      MN was characterized in human leukocytes treated with mitomycin C (MMC) and 
      bleomycin (BLM) by FISH using centromeric (cep) and whole-chromosome painting 
      (wcp) probes. Involvement of chromosomes 8, 15 and 20 in MMC-induced and 
      chromosomes 1, 9 and 16 in BLM-induced MN was studied, and correlated with 
      chromosome size, gene density and interphase position. The results obtained were 
      analyzed together with previous own data on the frequencies of inclusion of 
      chromosomes 3, 4, 6, 7, 9, 16, 17, 18, and X in MMC-induced MN. It could be shown 
      that MMC- and BLM-induced MN could contain material derived from all chromosomes 
      investigated. Involvement of whole chromosomes 8, 15 and 20 in MMC-induced MN 
      negatively correlated with gene density; however, analysis together with earlier 
      studied chromosomes did not confirm this correlation. Inclusion of chromosomes 8, 
      15 and 20 in MMC-induced MN does not depend on their size and interphase 
      position; the same result was found for the twelve overall analyzed chromosomes. 
      In BLM-treated cells significant correlation between frequencies of involvement 
      of chromosomes 1, 9 and 16 in MN and their size was found. CONCLUSIONS: Our 
      results clearly revealed that BLM differs from MMC with respect to the 
      distribution of induced chromosome damage and MN formation. Thus, DNA-damaging 
      agents with diverse mechanism of action induce qualitatively different MN with 
      regard to their chromosomal composition. Also this study demonstrates the utility 
      of combined sequential application of cep and wcp probes for efficient detection 
      of MN chromosomal content in terms of centric and acentric fragments.
FAU - Hovhannisyan, Galina
AU  - Hovhannisyan G
AUID- ORCID: 0000-0002-7450-1508
AD  - Department of Genetics and Cytology, Faculty of Biology, Yerevan State 
      University, 1 Alex Manoogian, 0025 Yerevan, Armenia.
FAU - Aroutiounian, Rouben
AU  - Aroutiounian R
AD  - Department of Genetics and Cytology, Faculty of Biology, Yerevan State 
      University, 1 Alex Manoogian, 0025 Yerevan, Armenia.
FAU - Babayan, Nelly
AU  - Babayan N
AD  - Department of Genetics and Cytology, Faculty of Biology, Yerevan State 
      University, 1 Alex Manoogian, 0025 Yerevan, Armenia ; Institute of Molecular 
      Biology, National Academy of Sciences, 7 Hasratyan, 0014 Yerevan, Armenia.
FAU - Harutyunyan, Tigran
AU  - Harutyunyan T
AD  - Department of Genetics and Cytology, Faculty of Biology, Yerevan State 
      University, 1 Alex Manoogian, 0025 Yerevan, Armenia.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
LA  - eng
PT  - Journal Article
DEP - 20160621
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC4915088
OTO - NOTNLM
OT  - Bleomycin
OT  - Chromosome
OT  - FISH
OT  - Human leukocytes
OT  - Micronuclei
OT  - Mitomycin C
EDAT- 2016/06/23 06:00
MHDA- 2016/06/23 06:01
PMCR- 2016/06/21
CRDT- 2016/06/23 06:00
PHST- 2016/04/19 00:00 [received]
PHST- 2016/06/15 00:00 [accepted]
PHST- 2016/06/23 06:00 [entrez]
PHST- 2016/06/23 06:00 [pubmed]
PHST- 2016/06/23 06:01 [medline]
PHST- 2016/06/21 00:00 [pmc-release]
AID - 258 [pii]
AID - 10.1186/s13039-016-0258-4 [doi]
PST - epublish
SO  - Mol Cytogenet. 2016 Jun 21;9:49. doi: 10.1186/s13039-016-0258-4. eCollection 
      2016.