PMID- 27330861
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160622
LR  - 20200930
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 4
DP  - 2016
TI  - Detection of antibiotic-resistant bacteria endowed with antimicrobial activity 
      from a freshwater lake and their phylogenetic affiliation.
PG  - e2103
LID - 10.7717/peerj.2103 [doi]
LID - e2103
AB  - Antimicrobial resistance poses a serious challenge to global public health. In 
      this study, fifty bacterial strains were isolated from the sediments of a 
      freshwater lake and were screened for antibiotic resistance. Out of fifty 
      isolates, thirty-three isolates showed resistance against at least two of the 
      selected antibiotics. Analysis of 16S rDNA sequencing revealed that the isolates 
      belonged to ten different genera, namely Staphylococcus(n = 8), Bacillus(n = 7), 
      Lysinibacillus(n = 4), Achromobacter(n=3), bacterium(n = 3), Methylobacterium(n = 
      2), Bosea(n = 2), Aneurinibacillus(n = 2), Azospirillum(n = 1), Novosphingobium(n 
      = 1). Enterobacterial repetitive intergenic consensus (ERIC) and BOX-PCR markers 
      were used to study the genetic relatedness among the antibiotic resistant 
      isolates. Further, the isolates were screened for their antimicrobial activity 
      against bacterial pathogens viz., Staphylococcus aureus(MTCC-96), Pseudomonas 
      aeruginosa(MTCC-2453) and Escherichia coli(MTCC-739), and pathogenic fungi viz., 
      Fusarium proliferatum (MTCC-286), Fusarium oxysporum (CABI-293942) and Fusarium 
      oxy. ciceri (MTCC-2791). In addition, biosynthetic genes (polyketide synthase II 
      (PKS-II) and non-ribosomal peptide synthetase (NRPS)) were detected in six and 
      seven isolates, respectively. This is the first report for the multifunctional 
      analysis of the bacterial isolates from a wetland with biosynthetic potential, 
      which could serve as potential source of useful biologically active metabolites.
FAU - Zothanpuia
AU  - Zothanpuia
AD  - Department of Biotechnology, Mizoram University , Aizawl , Mizoram , India.
FAU - Passari, Ajit K
AU  - Passari AK
AD  - Department of Biotechnology, Mizoram University , Aizawl , Mizoram , India.
FAU - Gupta, Vijai K
AU  - Gupta VK
AD  - Molecular Glyco-biotechnology Group, University of Ireland , Galway , Ireland , 
      UK.
FAU - Singh, Bhim P
AU  - Singh BP
AD  - Department of Biotechnology, Mizoram University , Aizawl , Mizoram , India.
LA  - eng
PT  - Journal Article
DEP - 20160609
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC4906672
OTO - NOTNLM
OT  - 16S rRNA gene
OT  - Antibiotic susceptibility
OT  - BOX-PCR
OT  - ERIC-PCR
OT  - NRPS
OT  - PKS II
COIS- Vijai K. Gupta is an Academic Editor for PeerJ.
EDAT- 2016/06/23 06:00
MHDA- 2016/06/23 06:01
PMCR- 2016/06/09
CRDT- 2016/06/23 06:00
PHST- 2016/03/14 00:00 [received]
PHST- 2016/05/11 00:00 [accepted]
PHST- 2016/06/23 06:00 [entrez]
PHST- 2016/06/23 06:00 [pubmed]
PHST- 2016/06/23 06:01 [medline]
PHST- 2016/06/09 00:00 [pmc-release]
AID - 2103 [pii]
AID - 10.7717/peerj.2103 [doi]
PST - epublish
SO  - PeerJ. 2016 Jun 9;4:e2103. doi: 10.7717/peerj.2103. eCollection 2016.