PMID- 27331018
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160622
LR  - 20220321
IS  - 2214-5400 (Print)
IS  - 2214-5400 (Electronic)
IS  - 2214-5400 (Linking)
VI  - 9
DP  - 2016 Sep
TI  - Tumor necrosis factor-alpha (TNF-alpha)-308G/A promoter polymorphism in colorectal cancer 
      in ethnic Kashmiri population - A case control study in a detailed perspective.
PG  - 128-36
LID - 10.1016/j.mgene.2016.06.001 [doi]
AB  - BACKGROUND: Inflammation constitutes one of the important components of 
      colorectal cancer (CRC) pathogenesis. Tumor necrosis factor-alpha (TNF-alpha), a cytokine 
      and an important inflammatory mediator plays a pivotal role in the malignant 
      cellular proliferation, angiogenesis, tissue invasion and metastasis in CRC. The 
      studies on association of various polymorphisms in human TNF-alpha gene including 
      TNF-alpha-308G/A single nucleotide polymorphism (SNP) are limited, mixed and 
      inconclusive. MATERIALS AND METHODS: The aim of this study was to analyze the 
      association of TNF-alpha-308G/A promoter SNP with colorectal cancer (CRC) 
      susceptibility and development risk and also to evaluate the modifying effects of 
      possible TNF-alpha-308G/A genotypes on different risk factors of CRC in ethnic 
      population of Kashmir, India through a case-control setup. The genotype 
      frequencies of TNF-alpha-308G/A promoter SNP were compared between 142 CRC patients 
      and 184 individually matched healthy controls by using polymerase chain reaction 
      and restriction fragment length polymorphism (PCR-RFLP) method. The associations 
      between the TNF-alpha-308G/A SNP and CRC risk were examined through conditional 
      logistic regression models adjusted for multiple possible confounding (third) 
      variables. Further, the associations between this SNP and various 
      clinico-pathological parameters, demographic variables and environmental factors 
      within the case group subjects with regard to CRC risk were also evaluated. 
      RESULTS: The association between the TNF-alpha-308G/A SNP and the modulation of risk 
      of CRC was not found to be significant (p value = 0.156). The effect of less 
      common TNF-alpha-308A allele on the risk of colorectal cancer was also not found to 
      be significant (p value = 0.175). The variant genotype (AA) was nonexistent in 
      the study population. Further, we found no significant effect modulation of CRC 
      risk by wild and heterozygous TNF-alpha-308G/A SNP genotypes in presence of different 
      possible risk factors (p > 0.05). We also found no significant association of 
      TNF-alpha-308G/A SNP with the subsets of various characteristics of the case group 
      subjects under study (p > 0.05). CONCLUSIONS: This study indicates that there is 
      no significant association between the TNF-alpha-308G/A promoter SNP and the risk of 
      developing CRC in ethnic Kashmiri population. However, in order to substantiate 
      our findings, this study needs to be replicated with bigger sample size and 
      should involve other ethnically defined populations with high CRC risk.
FAU - Banday, Mujeeb Zafar
AU  - Banday MZ
AD  - Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, Kashmir, 
      India.
FAU - Balkhi, Henah Mehraj
AU  - Balkhi HM
AD  - Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, Kashmir, 
      India.
FAU - Hamid, Zeenat
AU  - Hamid Z
AD  - Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, Kashmir, 
      India.
FAU - Sameer, Aga Syed
AU  - Sameer AS
AD  - Department of Basic Medical Sciences, College of Medicine, King Saud Bin 
      Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia.
FAU - Chowdri, Nissar A
AU  - Chowdri NA
AD  - Department of Surgery, Sher-I-Kashmir Institute of Medical Sciences, Soura, 
      Srinagar, Kashmir, India.
FAU - Haq, Ehtishamul
AU  - Haq E
AD  - Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, Kashmir, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20160603
PL  - Netherlands
TA  - Meta Gene
JT  - Meta gene
JID - 101627670
PMC - PMC4908285
OTO - NOTNLM
OT  - Case control study
OT  - Colorectal cancer (CRC)
OT  - Kashmir
OT  - Polymorphism
OT  - Single nucleotide polymorphism (SNP)
OT  - Tumor necrosis factor-alpha (TNF-alpha)
EDAT- 2016/06/23 06:00
MHDA- 2016/06/23 06:01
PMCR- 2016/06/03
CRDT- 2016/06/23 06:00
PHST- 2016/04/22 00:00 [received]
PHST- 2016/06/01 00:00 [accepted]
PHST- 2016/06/23 06:00 [entrez]
PHST- 2016/06/23 06:00 [pubmed]
PHST- 2016/06/23 06:01 [medline]
PHST- 2016/06/03 00:00 [pmc-release]
AID - S2214-5400(16)30018-4 [pii]
AID - 10.1016/j.mgene.2016.06.001 [doi]
PST - epublish
SO  - Meta Gene. 2016 Jun 3;9:128-36. doi: 10.1016/j.mgene.2016.06.001. eCollection 
      2016 Sep.