PMID- 27338695
OWN - NLM
STAT- MEDLINE
DCOM- 20161216
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1456
DP  - 2016 Jul 22
TI  - Ultrasensitive quantification of endogenous brassinosteroids in milligram fresh 
      plant with a quaternary ammonium derivatization reagent by pipette-tip 
      solid-phase extraction coupled with ultra-high-performance liquid chromatography 
      tandem mass spectrometry.
PG  - 105-12
LID - S0021-9673(16)30785-3 [pii]
LID - 10.1016/j.chroma.2016.06.026 [doi]
AB  - Determination of endogenous brassinosteroids (BRs) in limited sample amount is 
      vital to elucidating their tissue- and even local tissue-specific signaling 
      pathway and physiological effects on plant growth and development. In this work, 
      an ultra-sensitive quantification method was established for endogenous BRs in 
      milligram fresh plant by using pipette-tip solid-phase extraction coupled with 
      ultra-performance liquid chromatography tandem mass spectrometry 
      (PT-SPE-UPLC-MS/MS), in which a quaternary ammonium phenyl boronic acid, 
      4-borono-N,N,N-trimethylbenzenaminium iodide (BTBA) was first developed for 
      chemical derivatization of BRs. Due to the cationic quaternary ammonium group of 
      BTBA, the ionization efficiencies of the BRs chelates with BTBA (BTBA-BRs) were 
      enhanced by 1190-448785 times, which is the highest response enhancement factor 
      among all derivatization reagents reported for BRs. In addition, PT-SPE packed 
      with C18 sorbent was first used for purifying BRs from plant extracts, so the 
      required sample amount was minimized, and recoveries higher than 91% were 
      achieved. Under the optimized conditions, the minimal detectable amounts (MDA) of 
      five target BRs were in the range of 27-94 amol, and the correlation coefficients 
      (R(2)) were >0.9985 over four orders of magnitude. The relative recoveries of 
      75.8-104.9% were obtained with the intra- and inter-day relative standard 
      deviations (RSDs) less than 18.7% and 19.6%, respectively. Finally, three BRs 
      were successfully quantified in only 5mg fresh rice plant samples, and 24-epiBL 
      can even be detected in only 0.5mg FW rice leaf segments. It is the first time 
      that the BRs content in sub-milligram fresh plant sample has been quantified.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Deng, Ting
AU  - Deng T
AD  - Key Laboratory of Separation Science for Analytical Chemistry of CAS, Dept. of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China; 
      University of Chinese Academy of Sciences, Beijing 100039, PR China.
FAU - Wu, Dapeng
AU  - Wu D
AD  - Key Laboratory of Separation Science for Analytical Chemistry of CAS, Dept. of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China. 
      Electronic address: dpwu@dicp.ac.cn.
FAU - Duan, Chunfeng
AU  - Duan C
AD  - Key Laboratory of Separation Science for Analytical Chemistry of CAS, Dept. of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
FAU - Guan, Yafeng
AU  - Guan Y
AD  - Key Laboratory of Separation Science for Analytical Chemistry of CAS, Dept. of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China. 
      Electronic address: guanyafeng@dicp.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20160608
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Benzoates)
RN  - 0 (Brassinosteroids)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 24156-14-7 (2-benzylthio-5-trifluoromethylbenzoic acid)
SB  - IM
MH  - Benzoates/chemistry
MH  - Brassinosteroids/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Indicators and Reagents
MH  - Limit of Detection
MH  - Oryza/chemistry
MH  - Plant Leaves/chemistry
MH  - Plants/*chemistry
MH  - Quaternary Ammonium Compounds/*chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Brassinosteriod
OT  - Derivatization
OT  - Pipette-tip solid-phase extraction
OT  - Rice leaf segments
EDAT- 2016/06/25 06:00
MHDA- 2016/12/17 06:00
CRDT- 2016/06/25 06:00
PHST- 2016/04/09 00:00 [received]
PHST- 2016/06/07 00:00 [revised]
PHST- 2016/06/07 00:00 [accepted]
PHST- 2016/06/25 06:00 [entrez]
PHST- 2016/06/25 06:00 [pubmed]
PHST- 2016/12/17 06:00 [medline]
AID - S0021-9673(16)30785-3 [pii]
AID - 10.1016/j.chroma.2016.06.026 [doi]
PST - ppublish
SO  - J Chromatogr A. 2016 Jul 22;1456:105-12. doi: 10.1016/j.chroma.2016.06.026. Epub 
      2016 Jun 8.