PMID- 27340563
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160624
LR  - 20200930
IS  - 2090-004X (Print)
IS  - 2090-0058 (Electronic)
IS  - 2090-004X (Linking)
VI  - 2016
DP  - 2016
TI  - Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment 
      Epithelial Cells under Hypoxic Stress.
PG  - 9292346
LID - 10.1155/2016/9292346 [doi]
LID - 9292346
AB  - Aim. We investigated whether lecithin-bound iodine (LBI) can protect the 
      integrity of tight junctions of retinal pigment epithelial cells from hypoxia. 
      Method. Cultured human retinal pigment epithelial (ARPE-19) cells were pretreated 
      with LBI. To mimic hypoxic conditions, cells were incubated with CoCl2. We 
      compared the integrity of the tight junctions (TJs) of control to cells with 
      either LBI alone, CoCl2 alone, or LBI + CoCl2. The levels of cytokines in the 
      conditioned media were also determined. Results. Significant decrease in the 
      zonula occludens-1 (ZO-1) intensity in the CoCl2 group compared to the control 
      (5787.7 +/- 4126.4 in CoCl2 group versus 29244.6 +/- 2981.2 in control; average +/- 
      standard deviation). But the decrease was not significant in the LBI + CoCl2 
      (27189.0 +/- 11231.1). The levels of monocyte chemoattractant protein-1 (MCP-1) and 
      Chemokine (C-C Motif) Ligand 11 (CCL-11) were significantly higher in the CoCl2 
      than in the control (340.8 +/- 43.3 versus 279.7 +/- 68.3 pg/mL for MCP-1, and 15.2 +/- 
      12.9 versus 12.5 +/- 6.1 pg/mL for CCL-11. With LBI pretreatment, the levels of 
      both cytokines were decreased to 182.6 +/- 23.8 (MCP-1) and 5.46 +/- 1.9 pg/mL for 
      CCL-11). Blockade of MCP-1 or CCL-11 also shows similar result representing TJ 
      protection from hypoxic stress. Conclusions. LBI results in a protective action 
      from hypoxia.
FAU - Sugimoto, Masahiko
AU  - Sugimoto M
AD  - Department of Ophthalmology, Mie University, Graduate School of Medicine, 2-174 
      Edobashi, Tsu, Mie 514-8507, Japan.
FAU - Kondo, Mineo
AU  - Kondo M
AD  - Department of Ophthalmology, Mie University, Graduate School of Medicine, 2-174 
      Edobashi, Tsu, Mie 514-8507, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160531
PL  - United States
TA  - J Ophthalmol
JT  - Journal of ophthalmology
JID - 101524199
PMC - PMC4906216
EDAT- 2016/06/25 06:00
MHDA- 2016/06/25 06:01
PMCR- 2016/05/31
CRDT- 2016/06/25 06:00
PHST- 2016/02/25 00:00 [received]
PHST- 2016/05/06 00:00 [revised]
PHST- 2016/05/09 00:00 [accepted]
PHST- 2016/06/25 06:00 [entrez]
PHST- 2016/06/25 06:00 [pubmed]
PHST- 2016/06/25 06:01 [medline]
PHST- 2016/05/31 00:00 [pmc-release]
AID - 10.1155/2016/9292346 [doi]
PST - ppublish
SO  - J Ophthalmol. 2016;2016:9292346. doi: 10.1155/2016/9292346. Epub 2016 May 31.